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Chromosome Research

, Volume 7, Issue 6, pp 475–480 | Cite as

Chromosomal Characteristics of Ribosomal DNA in the Primitive Semionotiform Fish, Longnose Gar Lepisosteus osseus

  • P. Ráb
  • M. Rábová
  • K. M. Reed
  • R. B. Phillips
Article

Abstract

The chromosomes of longnose gar, Lepisosteus osseus, an extant representative of early radiation of actinopterygian fishes, were studied using conventional Giemsa-staining, Ag-staining, CMA3-fluorescence and fluorescence in-situ hybridization (FISH). The diploid chromosome number was 2n = 56 and the karyotype contained 11 pairs of metacentric, 6 pairs of submetacentric, 3 pairs of subtelocentric macrochromosomes and 16 microchromosomes. Nearly all macrochromosomes showed large CMA3-positive regions resembling the R-bands of higher vertebrates, indicating extensive distribution of GC-rich DNA along chromosomes. The nucleolar organizer regions (NORs) were located on the end of the short arm of a single small metacentric macrochromosomal pair. These sites were strongly CMA3-positive, suggesting that ribosomal sites are associated with GC- rich DNA. In-situ hybridization (FISH) with a rDNA probe gave consistently positive signals in the same regions detected by Ag- staining and CMA3-fluorescence. The evolutionary conservation of positive CMA3-fluorescence of ribosomal sites in ‘holostean’ and teleostean fishes is discussed.

chromomycin A3-banding FISH fish cytogenetics GC- rich heterochromatin longnose gar 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • P. Ráb
    • 1
  • M. Rábová
    • 1
  • K. M. Reed
    • 2
  • R. B. Phillips
    • 3
  1. 1.Laboratory of Fish GeneticsInstitute of Animal Physiology and Genetics, Czech Academy of SciencesLiběchovCzech Republic
  2. 2.Group in Comparative Genomics, Department of Veterinary PathoBiologyUniversity of MinnesotaSt. PaulUSA
  3. 3.Department of Biological SciencesUniversity of Wisconsin- MilwaukeeMilwaukeeUSA

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