Abstract
The nucleotide sequences of 280–360-bp domains of lectin genes from 20 legume species belonging to 17 genera have been determined. A computer analysis of the sequences has been performed with the LASERGENE package. Based on this analysis, we constructed the phylogenetic tree of the lectins, which reflects their phylogenetic and evolutionary relationships, and predicted the amino-acid sequences of the corresponding protein domains. Features of the structure of the hydrocarbon-binding lectin domains were elucidated in some species of legume genera from the temperate climatic zone. The domains were highly variable and contained the consensus sequence AspTrePheXxxAsxXxxXxxTrpAspProXxxXxxIns/DelArgHis bearing the bulk of amino acid replacements, insertions, and deletions. An association between legume groups (including species from different genera and tribes) symbiotic with the same rhizobium species and the similarity between the hydrocarbon-binding domains of lectins from these plants was found.
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REFERENCES
Rolfe, B. and Gresshoff, P.M., Genetic Analysis of Legume Nodule Initiation, Annu. Rev. Plant Physiol., 1988, vol. 39, pp. 297-319.
Provorov, N.A., Coevolution of Legumes and Nodule Bacteria: Taxonomic and Genetic Aspects, Zh. Obshch. Biol., 1996, vol. 57, no. 2, pp. 52-77.
Dazzo, F.B., Truchet, G.L., and Sherwood, J.E., Alteration of the Trifolin A-Binding Capsule of Rhizobium trifolii 0403 by Enzymes Released from Clover Roots, Appl. Environ. Microbiol., 1982, vol. 44, pp. 478-490.
Diaz, C.L., Melchers, L.S., Hooykaas, P.J.J., et al., Root Lectin as a Determinant of Host-Plant Specificity in the Rhizobium-Legume Symbiosis, Nature, 1989, vol. 338, pp. 579-581.
Kijne, J.W., Diaz, C.L., and Pater, S., Lectins in the Symbiosis between Rhizobia and Leguminous Plants, Advances in Lectin Research, Franz, H., van Driessche, E., and Kasai, K.J., Eds., Berlin: Ullstein Mosby, 1992, pp. 15-50.
Pariiskaya, A.N. and Klevenskaya, I.L., Distribution in Nature and Possible Ways of Evolution of Nitrogen-Fixation Symbiosis, Usp. Mikrobiol., 1979, vol. 14, pp. 124-147.
Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1989, 2nd ed.
Gatehouse, J.A., Bown, D., Evans, I.M., et al., Sequence of the Seed Lectin from Pea (Pisum sativum L.), Nucleic Acids Res., 1987, vol. 15, no. 18, p. 7642.
Foriers, A., de Neve, R., Kanarek, L., and Strosberg, A.D., Common Ancestor for Concanavalin A and Lentil Lectin?, Proc. Natl. Acad. Sci. USA, 1978, vol. 75, pp. 1136-1139.
Van Damme, E.J., Barre, A., Rouge, P., and Peumans, W.J., Molecular Cloning of the Bark and Seed Lectins from the Japanese Pagoda Tree (Sophora japonica), Plant Mol. Biol., 1997, vol. 33, no. 3, pp. 523-536.
Higgins, D.G. and Sharp, P.M., Fast and Sensitive Multiple Sequence Alignments on a Microcomputer, CABIOS, 1989, vol. 5, no. 2, pp. 151-153.
Takhtadzhyan, A.L., Sistema magnoliofitov (A System of Magnoliales), Leningrad: Nauka, 1987.
Konami, Y., Ishida, C., Yamamoto, K., and Irimura, T., A Unique Amino Acid Sequence Involved in the Putative Carbohydrate-Binding Domain of a Legume Lectin Specific for Sialylated Carbohydrate Chains: Primary Sequence Determination of Maackia amurensis Hemagglutinin (MAH), J. Biol. Chem., 1994, vol. 269, no. 13, pp. 9429-9435.
Rouge, P., Pere, D., and Bourne, Y., In Vitro Cleavage of the Lathyrus nissolia Isolectin, Plant Sci. (Limerick, Irel.), 1989, vol. 62, pp. 181-189.
Yamamoto, K., Konami, Y., and Osawa, T., Carbohydrate-Binding Peptides from Several Anti-H(O) Lectins, J. Biochem., 1992, vol. 111, pp. 436-439.
Konami, Y. and Yamamoto, K., Osawa T., Irimura T. The Primary Structure of the Cytisus scoparius Seed Lectin and a Carbohydrate-Binding Peptide, J. Biochem., 1992, vol. 112, pp. 366-375.
Loris, R., Hamelryck, T., Bouckaert, J., and Wyns, L., Legume Lectin Structure, Biochim. Biophys. Acta, 1998, vol. 1383, pp. 9-36.
Lakhtin, V.M., Molecular Structure of Lectins, Mol. Biol. (Moscow), 1994, vol. 28, no. 2, pp. 245-273.
Young, N.M. and Oomen, R.P., Analysis of Sequence Variation among Legume Lectins: A Ring of Hypervariable Residues Forms the Perimeter of the Carbohydrate-Binding Site, J. Mol. Biol., 1992, vol. 228, pp. 924-934.
van Eijsden, R.R., Hoedemaeker, F.J., and Diaz, C.L., Mutational Analysis of Pea Lectin: Substitution of Asn125 for Asp in the Monosaccharide-Binding Site Eliminates Mannose/Glucose-Binding Activity, Plant Mol. Biol., 1992, vol. 20, pp. 1049-1058.
Firmin, J.L., Wilson, K.E., Carlson, R.W., et al., Resistance to Nodulation of Cv Afghanistan Peas Is Overcome by NodX, Which Mediates an O-Acetylation of the Rhizobium leguminosarum Lipooligosaccharide Nodulation Factor, Mol. Microbiol., 1993, vol. 10, pp. 351-360.
Moreira-Silva, L.I.M., Sanz, L., Cavada, B.S., and Calvete, J.J., Characterization and Structure of a Lectin from Pisum arvense Seeds, Eur. J. Cell Biol., 1997, vol. 74, suppl. 46, p. 7.
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Baiymiev, A.K., Chemeris, A.V., Baiymiev, A.K. et al. Hydrocarbon-Binding Peptides from Legume Lectins in Relation to Different Host Specificity upon Legume–Rhizobium Symbiosis. Russian Journal of Genetics 37, 156–161 (2001). https://doi.org/10.1023/A:1009089805347
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DOI: https://doi.org/10.1023/A:1009089805347