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Effect of the Integration of Vector pJFF350 into Plasmid 85-MDa of Azospirillum brasilense Sp245 on Bacterial Flagellation and Motility

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Abstract

Results of genetic analysis of three derivatives of Azospirillum brasilense Sp245 (strains BK570, SK051, and SK248) carrying cointegrates of plasmids 85-MDa and pJFF350 (the vector for omegon mutagenesis), which manifest abnormalities in flagellation and motility, are presented. It was shown for the first time that the integration of the suicide vector into one of Azospirillum resident plasmids is accompanied by the formation of various fusion products and changes in flagellation and motility of these bacteria, such as the loss of the polar (Fla) and lateral (Laf) flagella in SK051; inactivation of Fla and Laf in SK248; and Fla-dependent acceleration of expansion in semiliquid media in BK570.

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REFERENCES

  1. Krieg, N.R. and Döbereiner, J., Genus Azospirillum (Tarrand, Krieg and Döbereiner, 1979), Bergey's Manual of Systematic Bacteriology, Krieg, N.R. and Holt, J.G., Eds., London: Williams and Wilkins, 1984, pp. 94-104.

    Google Scholar 

  2. Hall, P.G. and Krieg, N.R., Swarming of Azospirillum brasilense on Solid Media, Can. J. Microbiol., 1983, vol. 29, pp. 1592-1594.

    Google Scholar 

  3. Moens, S., Michiels, K., Keijers, V., et al., Cloning, Sequencing, and Phenotypic Analysis of laf1 Encoding Flagellin of the Lateral Flagella of Azospirillum brasilense Sp7, J. Bacteriol., 1995, vol. 177, pp. 5419-5426.

    Google Scholar 

  4. Moens, S., Schloter, M., and Vanderleyden, J., Expression of the Structural Gene, laf1, Encoding the Flagellin of the Lateral Flagella in Azospirillum brasilense Sp7, J. Bacteriol, 1996, vol. 178, pp. 5017-5019.

    Google Scholar 

  5. Scheludko, A.V., Katzy, E.I., Ostudin, N.A., et al., Novel Classes of Azospirillum brasilense Mutants with Defects in the Assembly and Functioning of Polar and Lateral Flagella, Mol. Genet., Mikrobiol. Virusol., 1998, no. 4, pp. 33-37.

  6. Caballero-Mellado, J., Lopez-Reyes, L., and Bustillos-Cristales, R., Presence of 16S rRNA Genes in Multiple Replicons in Azospirillum brasilense, FEMS Microbiol. Lett., 1999, vol. 178, pp. 283-288.

    Google Scholar 

  7. Katzy, E.I., Iosipenko, A.D., Egorenkov, D.A., et al., Involvement of the Azospirillum brasilense Plasmid DNA in Indole Acetic Acid Production, FEMS Microbiol. Lett., 1990, vol. 72, pp. 1-4.

    Google Scholar 

  8. Croes, C., Van Bastelaere, E., DeClerq, E., et al., Identification and Mapping of Loci Involved in Motility, Adsorption to Wheat Roots, Colony Morphology, and Growth in Minimal Medium on the Azospirillum brasilense Sp7 90-MDa Plasmid, Plasmid, 1991, vol. 26, pp. 83-93.

    Google Scholar 

  9. Katzy, E.I., Matora, L.Yu., Serebrennikova, O.B., and Scheludko, A.V., Involvement of a 120-MDa Plasmid of Azospirillum brasilense Sp245 in the Production of Lipopolysaccharides, Plasmid, 1998, vol. 40, pp. 73-83.

    Google Scholar 

  10. Katzy, E.I. and Scheludko, A.V., Mapping of the fla Locus to a 120-MDa Plasmid in Bacterium Azospirillum brasilense Sp245, Genetika (Moscow), 1999, vol. 35, no. 10, pp. 1367-1372.

    Google Scholar 

  11. Moens, S., Michiels, K., and Vanderleyden, J., Glycosylation of the Flagellin of the Polar Flagellum of Azospirillum brasilense, a Gram-Negative Nitrogen-Fixing Bacterium, Microbiology, 1995, vol. 141, pp. 2651-2657.

    Google Scholar 

  12. Hanahan, D., Studies on Transformation of Escherichia coli with a Plasmid, J. Mol. Biol., 1983, vol. 166, pp. 557-580.

    Google Scholar 

  13. Simon, R., Priefer, U., and Pühler, A., A Broad Host Range Mobilization System for in Vivo Genetic Engineering: Transposon Mutagenesis in Gram-Negative Bacteria, Bio/Technology, 1983, vol. 1, pp. 784-791.

    Google Scholar 

  14. Baldani, V.L.D., Baldani, J.I., and Döbereiner, J., Effects of Azospirillum Inoculation on Root Infection and Nitrogen Incorporation in Wheat, Can. J. Microbiol., 1983, vol. 29, pp. 924-929.

    Google Scholar 

  15. Fellay, R., Krisch, H.M., Prentki, P., and Frey, J., Omegon-Km: A Transposable Element Designed for in Vivo Insertional Mutagenesis and Cloning of Genes in Gram-Negative Bacteria, Gene, 1989, vol. 76, pp. 215-226.

    Google Scholar 

  16. Döbereiner, J. and Day, J.M., Associative Symbiosis in Tropical Grasses: Characterization of Microorganisms and Dinitrogen Fixing Sites, Int. Symp. on Nitrogen Fixation--Interdisciplinary Discussion, Newton, W.E. and Nijmans, C.J., Eds., Washington: Washington Univ., 1976, vol. 2, pp. 518-538.

    Google Scholar 

  17. Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1989, vols. 1-3.

    Google Scholar 

  18. Eckhardt, T., A Rapid Method for the Identification of Plasmid Deoxyribonucleic Acid in Bacteria, Plasmid, 1978, vol. 1, pp. 584-588.

    Google Scholar 

  19. Wood, C.C., Ritchie, R.J., and Kennedy, I.R., Membrane Potential, Proton and Sodium Motive Forces in Azospirillum brasilense Sp7-S, FEMS Microbiol. Lett., 1998, vol. 164, pp. 295-301.

    Google Scholar 

  20. Matora, L., Sumaroka, M., Dykman, L., et al., Revealing a Cap on the Polar Flagellum of Azospirillum brasilense Sp245, Abstracts 12th Int. Congr. on Nitrogen Fixation, Parana, Brazil, 1999, p. 99.

  21. Katzy, E.I., Borisov, I.V., Mashkina, A.B., and Panasenko, V.I., Effect of the Plasmid Composition on the Chemotactic Reaction of Cereal-Associated Bacterium Azospirillum brasilense Sp245, Mol. Genet., Mikrobiol. Virusol., 1994, no. 2, pp. 29-32.

  22. Katzy, E.I., Zhuravleva, E.A., and Panasenko, V.I., Transposon Mutagenesis and Plasmid Elimination and Mobilization in Nitrogen-Fixing Bacterium Azospirillum brasilense Sp245, Mol. Genet., Mikrobiol. Virusol., 1990, no. 2, pp. 29-32.

  23. Michiels, K., Vanstockem, M., Vanderleyden, J., and Van Gool, A., Stability of Broad Host Range Plasmids in Azospirillum: Cloning of a 5.9-Kb Plasmid from Azospirillum brasilense R07, Azospirillum III: Genetics–Physiology–Ecology, Klingmuller, W., Ed., Berlin: Springer-Verlag, 1985, pp. 63-73.

    Google Scholar 

  24. Macnab, R.M., Genetics and Biogenesis of Bacterial Flagella, Annu. Rev. Genet., 1992, vol. 26, pp. 131-158.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, 410015, Russia

    E. I. Katzy, I. V. Borisov & A. V. Scheludko

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  1. E. I. Katzy
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  2. I. V. Borisov
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  3. A. V. Scheludko
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Katzy, E.I., Borisov, I.V. & Scheludko, A.V. Effect of the Integration of Vector pJFF350 into Plasmid 85-MDa of Azospirillum brasilense Sp245 on Bacterial Flagellation and Motility. Russian Journal of Genetics 37, 129–134 (2001). https://doi.org/10.1023/A:1009081603530

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