Abstract
Three mutants of Azospirillum brasilense Sp245 incapable of both formation of the polar flagellum (Fla–-phenotype) and swarming in semisolid media (Swa–-phenotype) were characterized. These mutants were shown to have lost the 85-MDa plasmid and to carry the Tn5-Mob transposon and pSUP5011 vector in different regions of their genomes. With the use of A. brasilense Sp245 gene bank, the capacity for both polar flagellum formation and swarming was restored in the above mutants and in the previously generated transposon mutants A. brasilense Sp245 and S27. The transconjugants obtained were only slightly motile in the liquid culture. In the gene bank of Sp245, the recombinant plasmids carrying wild-type fla/swa loci were identified.
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REFERENCES
Katzy, E.I., Genetic, Biochemical, and Ecological Aspects of Motility and Chemotaxis in Phytopathogenic, Symbiotic, and Plant-Associated Bacteria, Usp. Sovrem. Biol., 1996, vol. 116, no. 5, pp. 579-593.
Hall, P.G. and Krieg, N.R., Swarming of Azospirillum brasilense on Solid Media, Can. J. Microbiol., 1983, vol. 29, pp. 1592-1594.
Harshey, R.M., Bees Aren't the Only Ones: Swarming in Gram-Negative Bacteria, Mol. Microbiol., 1994, vol. 13, pp. 389-394.
McCarter, L.L., The Multiple Identities of Vibrio parahaemolyticus, J. Mol. Microbiol. Biotechnol., 1999, vol. 1, pp. 51-57.
Scheludko, A.V., Katzy, E.I., Ostudin, N.A., et al., Novel Classes of Azospirillum brasilense Mutants with Defects in the Assembly and Functioning of Polar and Lateral Flagella, Mol. Genet., Mikrobiol. Virusol., 1998, no. 4, pp. 33-37.
Katzy, E.I. and Scheludko, A.V., Mapping of the fla Locus to a 120-MDa Plasmid in Azospirillum brasilense Sp245, Genetika (Moscow), 1999, vol. 35, no. 10, pp. 1367-1372.
Moens, S., Michiels, K., Keijers, V., et al., Cloning, Sequencing, and Phenotypic Analysis of laf1 Encoding Flagellin of the Lateral Flagella of Azospirillum brasilense Sp7, J. Bacteriol., 1995, vol. 177, pp. 5419-5426.
Katzy, E.I., Matora, L.Yu., Serebrennikova, O.B., and Scheludko, A.V., Involvement of a 120-MDa Plasmid of Azospirillum brasilense Sp245 in the Production of Lipopolysaccharides, Plasmid, 1998, vol. 40, pp. 73-83.
Croes, C., Van Bastelaere, E., DeClerq, E., et al., Identification and Mapping of Loci Involved in Motility, Adsorption to Wheat Roots, Colony Morphology, and Growth in Minimal Medium on the Azospirillum brasilense Sp7 90-MDa Plasmid, Plasmid, 1991, vol. 26, pp. 83-93.
Baldani, V.L.D., Baldani, J.I., and Döbereiner, J., Effects of Azospirillum Inoculation on Root Infection and Nitrogen Incorporation in Wheat, Can. J. Microbiol., 1983, vol. 29, pp. 924-929.
Katzy, E.I., Zhuravleva, E.A., and Panasenko, V.I., Transposon-Induced Mutagenesis and Plasmid Elimination and Mobilization in Nitrogen-Fixing Bacterium Azospirillum brasilense Sp245, Mol. Genet., Mikrobiol. Virusol., 1990, no. 2, pp. 29-32.
Katzy, E.I. and Scheludko, A.V., Mutagenesis Induced by the TnphoA Transposon as a Means to Obtain Azospirillum brasilense Mutants Defective in Motility and Flagellation, Mol. Genet., Mikrobiol. Virusol., 2000, no. 4, pp. 17-20.
Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1989, 2nd ed, vol. 1-3.
Simon, R., High-Frequency Mobilization of Gram-Negative Bacterial Replicons by the in Vivo Constructed Tn5-Mob Transposon, Mol. Gen. Genet., 1984, vol. 196, pp. 413-420.
Simon, R., Priefer, U., and Pöhler, A., A Broad Host Range Mobilization System for in Vivo Genetic Engineering: Transposon Mutagenesis in Gram-Negative Bacteria, Bio/Technology, 1983, vol. 1, pp. 784-791.
Saano, A.K., Construction of Vector Systems for Transformation of Photosynthetic Bacteria Rhodopseudomonas sphaeroides, Cand. Sci. (Biol.) Dissertation, Moscow: Moscow State Univ., 1985.
Figurski, D.H. and Helinski, D.R., Replication of an Origin-Containing Derivative of Plasmid RK2 Dependent on a Plasmid Function Provided in Trans, Proc. Natl. Acad. Sci. USA, 1979, vol. 76, pp. 1648-1652.
Döbereiner, J. and Day, J.M., Associative Symbiosis in Tropical Grasses: Characterization of Microorganisms and Dinitrogen Fixing Sites, Int. Symp. on Nitrogen Fixation-Interdisciplinary Discussion, Newton, E. and Nijman, C.J., Eds., Washington: Washington Univ., 1976, vol. 2, pp. 518-538.
Eckhardt, T., A Rapid Method for the Identification of Plasmid Deoxyribonucleic Acid in Bacteria, Plasmid, 1978, vol. 1, pp. 191-197.
Katzy, E.I., Borisov, I.V., Mashkina, A.B., and Panasenko, V.I., Effect of Plasmid Composition on the Chemotactic Reactions of Cereal-Associated Bacterium Azospirillum brasilense Sp245, Mol. Genet., Mikrobiol. Virusol., 1994, no. 2, pp. 29-32.
Katzy, E.I., Borisov, I.V., and Scheludko, A.V., Effect of the Integration of Vector pJFF350 into a 85-MDa Plasmid on Flagellation and Motility of Azospirillum brasilense Sp245, Genetika (Moscow), 2001, vol. 37, no. 2, pp. 191-197.
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Kamneva, A.B., Katzy, E.I., Borisov, I.V. et al. Complementation Analysis of Associative Bacteria Azospirillum brasilense Sp245 and S27 Defective for Motility and Flagellation. Russian Journal of Genetics 37, 135–140 (2001). https://doi.org/10.1023/A:1009033720368
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DOI: https://doi.org/10.1023/A:1009033720368