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Degradation of polychlorinated biphenyls by extracellular enzymes of Phanerochaete chrysosporium produced in a perforated plate bioreactor

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Abstract

The white rot fungus Phanerochaete chrysosporium was cultivated in a perforated plate bioreactor and the expression of activities of manganese-dependent peroxidase (MnP) and lignin peroxidase (LiP) was measured. Peak activities of the two enzymes were reached close to day 11 and therefore the cultivation was terminated on that day. Extracellular proteins were concentrated and both peroxidases separated by isoelectric focusing. Degradation of technical PCB mixtures containing low and highly chlorinated congeners (Delor 103 and Delor 106 as equivalents of Aroclor 1242 and Aroclor 1260, respectively) was performed using intact mycelium, crude extracellular liquid and enriched MnP and LiP. A decrease in PCB concentration caused by a 44-h treatment with mycelium (74% w/w for Delor 103 and 73% for Delor 106) or crude extracellular liquid (62% for Delor 103 and 58% for Delor 106) was observed. The degradation was not substrate-specific, because no significant differences between the respective degradation rates were observed with di-, tri-, tetra-, penta-, hexa-, hepta-, and octachlorinated congeners. In contrast, MnP and LiP isolated from the above-mentioned extracellular liquid did not catalyse any degradation.

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Authors and Affiliations

  1. Veterinary Research Institute, 621 32, Brno, Czech Republic

    Pavel Krčmář

  2. Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic

    Alena Kubátová, Jaroslav Votruba, Pavla Erbanová, Čeněk Novotný & Václav Šašek

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  1. Pavel Krčmář
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  2. Alena Kubátová
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  3. Jaroslav Votruba
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  4. Pavla Erbanová
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  5. Čeněk Novotný
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  6. Václav Šašek
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Krčmář, P., Kubátová, A., Votruba, J. et al. Degradation of polychlorinated biphenyls by extracellular enzymes of Phanerochaete chrysosporium produced in a perforated plate bioreactor. World Journal of Microbiology and Biotechnology 15, 269–276 (1999). https://doi.org/10.1023/A:1008994912875

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