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Development of a shuttle vector for screening of strong promoter sequences

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Biotechnology Techniques

Abstract

A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.

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Authors and Affiliations

  1. Biological Sciences Department, Heriot-Watt University, Riccarton, Edinburgh, EH14 4AS, Scotland

    Martin Tangney & Wilfrid J. Mitchell

  2. Department of Bacterial Gene Technology, Novo Nordisk A/S, Novo Alle, DK-2880, Bagsv\sgmaelig;rd, Denmark

    Michael Dolberg-Rasmussen

Authors
  1. Martin Tangney
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  2. Wilfrid J. Mitchell
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  3. Michael Dolberg-Rasmussen
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Tangney, M., Mitchell, W.J. & Dolberg-Rasmussen, M. Development of a shuttle vector for screening of strong promoter sequences. Biotechnology Techniques 13, 141–144 (1999). https://doi.org/10.1023/A:1008983208118

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  • DOI: https://doi.org/10.1023/A:1008983208118

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