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Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response

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Abstract

Respiratory syncytial virus (RSV) is one of the most important pathogens of infancy and early childhood. Here a fruit-based edible subunit vaccine against RSV was developed by expressing the RSV fusion (F) protein gene in transgenic tomato plants. The F-gene was expressed in ripening tomato fruit under the control of the fruit-specific E8 promoter. Oral immunization of mice with ripe transgenic tomato fruits led to the induction of both serum and mucosal RSV-F specific antibodies. The ratio of immunoglobulin subclasses produced in response to immunization suggested that a type 1 T-helper cell immune response was preferentially induced. Serum antibodies showed an increased titer when the immunized mice were exposed to inactivated RSV antigen.

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Authors and Affiliations

  1. Department of Molecular and Integrative Physiology, Urbana, IL, 61801

    Jagdeep S. Sandhu & Mark D. Osadjan

  2. Department of Natural Resources and Environment Sciences, Urbana, IL, 61801

    Sergei F. Krasnyanski & Schuyler S. Korban

  3. United States Department of Agriculture, Agricultural Research Service and Department of Crop Sciences, University of Illinois, Urbana, IL, 61801

    Leslie L. Domier

  4. Department of Molecular and Integrative Physiology, Urbana, IL, 61801

    Dennis E. Buetow

Authors
  1. Jagdeep S. Sandhu
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  2. Sergei F. Krasnyanski
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  3. Leslie L. Domier
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  4. Schuyler S. Korban
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  5. Mark D. Osadjan
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  6. Dennis E. Buetow
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Sandhu, J.S., Krasnyanski, S.F., Domier, L.L. et al. Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response. Transgenic Res 9, 127–135 (2000). https://doi.org/10.1023/A:1008979525909

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  • DOI: https://doi.org/10.1023/A:1008979525909