Biotechnology Techniques

, Volume 13, Issue 7, pp 463–467 | Cite as

Affinity chromatography using enzymatically synthesized nucleotide-containing DNA binding polymers

  • Qun Wang
  • Robert J. Linhardt
  • Jonathan S. Dordick

Abstract

A series of nucleotide-containing polyphenols has been synthesized by a simple, two-step enzymatic method. The binding properties of these synthetic polymers to complementary oligonucleotides have been evaluated using a commercially available oligo(dT)cellulose column. Complementary synthetic nucleosides were retained on this column to a greater extent than non-complementary synthetic nucleosides. These results suggest that the synthetic nucleosides prepared via this two-step enzymatic approach may have application as affinity matrices.

affinity chromatography DNA binding synthetic nucleosides 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Qun Wang
    • 1
  • Robert J. Linhardt
    • 1
  • Jonathan S. Dordick
    • 2
  1. 1.Division of Medicinal and Natural Product Chemistry and Department of Chemical and Biochemical EngineeringThe University of IowaIowa CityUSA
  2. 2.Department of Chemical EngineeringRensselaer Polytechnic InstituteTroyUSA

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