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Affinity chromatography using enzymatically synthesized nucleotide-containing DNA binding polymers

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Abstract

A series of nucleotide-containing polyphenols has been synthesized by a simple, two-step enzymatic method. The binding properties of these synthetic polymers to complementary oligonucleotides have been evaluated using a commercially available oligo(dT)cellulose column. Complementary synthetic nucleosides were retained on this column to a greater extent than non-complementary synthetic nucleosides. These results suggest that the synthetic nucleosides prepared via this two-step enzymatic approach may have application as affinity matrices.

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Correspondence to Jonathan S. Dordick.

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Wang, Q., Linhardt, R.J. & Dordick, J.S. Affinity chromatography using enzymatically synthesized nucleotide-containing DNA binding polymers. Biotechnology Techniques 13, 463–467 (1999). https://doi.org/10.1023/A:1008977412471

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  • affinity chromatography
  • DNA binding
  • synthetic nucleosides