Affinity chromatography using enzymatically synthesized nucleotide-containing DNA binding polymers
- 37 Downloads
A series of nucleotide-containing polyphenols has been synthesized by a simple, two-step enzymatic method. The binding properties of these synthetic polymers to complementary oligonucleotides have been evaluated using a commercially available oligo(dT)cellulose column. Complementary synthetic nucleosides were retained on this column to a greater extent than non-complementary synthetic nucleosides. These results suggest that the synthetic nucleosides prepared via this two-step enzymatic approach may have application as affinity matrices.
Unable to display preview. Download preview PDF.
- Aviv H, Leder P (1972) Proc. Natl. Acad. Sci. USA 69: 1408-1412.Google Scholar
- Garcia R, Pizzi A (1998) J. Appl. Polym. Sci. V7: 1111-1119.Google Scholar
- Liu R, Orgel LE (1995) Nucl. Acids Res. 23: 3742-3749.Google Scholar
- Ludwick AG, Robinson KS, Mccloud J, Jr (1986) J. Polym. Sci. Polym. Symp. 74: 55-69.Google Scholar
- Mag M, Muth J, Jahn K, Peyman A, Krethzsxhmar G, Engels JW, Uhlmann E (1997) Bioorg. Med. Chem. 5: 2213-2220.Google Scholar
- Noe CR, Windhab N, Haberhauer G (1995) Arch. Pharm. 328: 743-744.Google Scholar
- Overberger CG, Chang JY (1989) J. Polym. Sci. Polym. Chem. 27: 3589-3602.Google Scholar
- Rich JO, Dordick JS (1997) J. Am. Chem. Soc. 119: 3234-3252.Google Scholar
- Thanki G, Beach TA, Dickerman HW (1978) J. Biol. Chem. 253: 7744-7750.Google Scholar
- Uhlmann E, Peyman A (1990) Chem. Rev. 90: 544-579.Google Scholar
- Wang P, Dordick JS (1998) Macromolecules: 31: 941-943.Google Scholar