Synthetic collagen fibers coated with a synthetic peptide containing the YIGSR sequence of laminin to promote peripheral nerve regeneration in vivo

  • S. Itoh
  • K. Takakuda
  • H. Samejima
  • T. Ohta
  • K. Shinomiya
  • S. Ichinose


The usefulness of collagen fibers and the YIGSR sequence (Tyr-lle-Gly-Ser-Arg) of laminin for nerve regeneration were examined in vivo. Type I collagen gel (G-group), Type I collagen fibers (F-group), Type I collagen fibers coated with laminin (L-group) or the YIGSR sequence (Y-group) were packed into silicone tubes, 15 mm long, and transplanted to the sciatic nerves of Wistar rats. Empty silicone tubes were used as the control. The animals were sacrificed 8 weeks after transplantation. Bridging of the nerve was confirmed in the F-(7/12), Y-(7/10) and L-group (6/10), but no bridging was observed in any of the animals of the G- and control group. Nerve regeneration among the space of collagen fibers was observed, and it was suggested that fibroblasts infiltrated the gap in the substance of the degenerated collagen fibers were followed by Schwann cells on the basis of immunocytochemistry. The number of myelinated axons per regenerated tissue in the tube (density), and total area of myelinated axons per measured regenerated tissue in the tube (% axon area) in each the L- and Y-group were significantly higher than that in the F-group (P < 0.05). These results suggest the possibility of obtaining adequate nerve regeneration with new artificial materials only. © 1999 Kluwer Academic Publishers


Collagen Polymer Peptide Peripheral Nerve Laminin 
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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • S. Itoh
    • 1
  • K. Takakuda
    • 2
  • H. Samejima
    • 1
  • T. Ohta
    • 1
  • K. Shinomiya
    • 1
  • S. Ichinose
    • 3
  1. 1.Department of Orthopaedic SurgeryTokyo Medical and Dental UniversityTokyoJapan
  2. 2.Division of Applied Mechanics Institute for Medical and Dental EngineeringTokyo Medical and Dental UniversityTokyoJapan
  3. 3.Center for Electron MicroscopyTokyo Medical and Dental UniversityTokyoJapan

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