Abstract
A rapid, continuous, colorimetric enzyme assay for penicillin G acylase has been developed. The assay measures the formation of the acidic products of penicillin G hydrolysis by following the decrease in pH using Phenol Red as an indicator. The activity measured is directly proportional to the amount of enzyme added to the assay, having a linear relationship with an R 2 value of 0.9994.
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Simons, H., Gibson, T.D. Rapid continuous colorimetric enzyme assay for penicillin G acylase. Biotechnology Techniques 13, 365–367 (1999). https://doi.org/10.1023/A:1008975600792
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DOI: https://doi.org/10.1023/A:1008975600792