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Evaluation of cell numbers and viability of Saccharomyces cerevisiae by different counting methods

Abstract

Four counting methods (two flow cytometry, one Coulter principle, one microscopic) are compared for measuring cell density and viability of batch-grown yeast. All gave adequate precision in measuring total cell density with no systematic difference between methods. However, the promise of flow cytometry as a rapid means of determining both quantity and quality of a cell population is shown.

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Fiala, J., Lloyd, D., Rychtera, M. et al. Evaluation of cell numbers and viability of Saccharomyces cerevisiae by different counting methods. Biotechnology Techniques 13, 787–795 (1999). https://doi.org/10.1023/A:1008974419342

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  • DOI: https://doi.org/10.1023/A:1008974419342

  • cell number
  • Coulter principle
  • flow cytometry
  • viability
  • yeast