Abstract
A high lysine-producing mutant of Lactobacillus plantarum (OG 261-5) derived from a wild type strain (OG 261) previously isolated from fermenting ogi was evaluated for nutritional improvement of ogi in a modified fermentation process. Results indicate that ogi obtained by fermentation with the pure culture of the mutant compared to traditional ogi increased in concentration of available lysine from 228.5 ± 12.0 mg/100 g to 525.1 ± 25.8 mg/100 g, tryptophan from 58.6 ± 8.0 mg/100 g to 114.3 ± 11.0 mg/100 g and tyrosine from 408.5 ± 13.7 mg/100 g to 4636.5 ± 11.3 mg/100 g. However, the contents of valine, leucine, isoleucine and phenylalanine were substantially reduced which may affect the protein quality of the modified ogi. The modified process shortened the period of traditional ogi production from five days to one by reducing the two-stage fermentation (i.e. soaking and souring stages) to a one-stage process and the total protein recovery was better compared to traditional ogi processing. There was no significant difference (P > 0.05) in organoleptic quality attributes of colour, flavour, and overall acceptability of ogi produced by the modified process and the traditional ogi.
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References
Achinewhu, S.C. 1987 Protein quality evaluation of weaning food mixtures from indigenous fermented foods. Nigerian Journal of Nutritional Sciences 8, 23-31.
Adebawo, O.O., Ruiz-Barba, J.L., Warner, P.J. & Oguntimein, G.B.O. 1997 Regulation of aspartokinase in Lactobacillus plantarum. Journal of Applied Microbiology 82, 191-196.
Adeyele, S. 1986 Microbiological studies of guinea corn fermentation for "Ogibaba" production. Ph.D. thesis, 143 pp. University of Ibadan, Nigeria.
Akingbala, J.O., Rooney, L.W. & Faubion, J.M. 1981 A laboratory procedure for the preparation of ogi. A Nigerian fermented food. Journal of Food Science 46, 1523-1526.
Akinrele, I.A., Adeyinka, O., Edwards, C.C.A., Olatunji, F.O., Aina, J.A. & Koleoso, O.A. 1970 The development and production of soy-ogi (A corn-based complete protein food). Federal Institute of Industrial Research, Oshodi Research Report No. 42.
Akobundu, E.N.T. & Hoskins, F.H. 1982 Protein losses in traditional agidi paste production. Journal of Food Science 47, 1728-1729.
Anon. 1985 Dehydrated Culture Media and Reagents for Microbiology. Difco Manual for Microbiological Culture Media, 10th edn, pp. 1055-1066: MI Difco Laboratories Inc. ISBN 0-9613169-9-3.
Association of Official Analytical Chemists (AOAC) 1985 Official Methods of Analysis, 14th edn ed. William Horwitz. pp. 137-232. Washington, DC: AOAC. ISBN 0-93558424-2.
Bangio, E.O.I. & Muller, H.G. 1972 Carboxylic acid pattern in ogi fermentation. Journal of Science of Food and Agriculture 23, 101-111.
Banigo, E.O.I., Deman, J.M. & Duitschaever, C.L. 1974 Utilization of high lysine corn for the manufacture of ogi using a new improved processing system. Cereal Chemistry 51, 559-572.
Bottazzi, V. 1985 An introduction to rod shaped lactic acid bacteria. Biochimie 70, 303-315.
Chassy, B.M. 1986 Lactobacilli in food fermentation. In: Biotechnology in Food Processing, eds. Hardlander, S.K. & Labuza, T.P. pp. 197-297. Park Ridge, New Jersey: Noyes Publications. ISBN 0-81551073-X.
Egounlety, T.E. & Syarief, R. 1992 Study on the supplementation of ogi with tempeh. Nigeria Food Journal 10, 92-102.
Flodin, N.W. 1993 Lysine supplementation of cereal foods: a retrospective. Journal of American College of Nutrition 12, 619-625.
Food and Drug Administration (FDA) 1984 Bacteriological Analytical Manual (B.A.M). pp 4.01-4.10. Arlington, USA: Association of Official Analytical Chemists (AOAC).
Haynes, P.A., Sheumack, D., Kibby, J. & Redmond, J.W. 1991 Amino acid analysis using derivatisation with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography. Journal of Chromatography 540, 177-185.
International Crops Research Institute for the Semi-arid Tropics (ICRISAT) 1991 Crop Quality Unit Laboratory Manual. p. 19. Patancheru, Andhra Pradesh (502324), India.
Larmond, E. 1937 Laboratory methods for sensory evaluation of foods. Department of Agriculture Publication No. 1637, Ottawa, Canada.
Lay, M.M. & Fields, M.L. 1981 Nutritive value of germinated corn and corn fermented after germination. Journal of Food Science 46, 1069-1073.
Ledesma, O.V., Avda, P., Holgado, R., Oliver, G., Degiori, G.S., Raiband, P. & Galpin, J.V. 1977 A synthetic medium for comparative nutritional studies of lactobacilli. Journal of Applied Bacteriology 42, 123-133.
Mertz, E.T., Batesand, L.S. & Nelson, O.E. 1964 Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145, 279-280.
Morishita, T., Deguchi, Y., Yajima, M., Sakurai, T. & Yura, T. 1981 Multiple nutritional requirements of Lactobacillus: Geneic lessions affecting amino acid biosynthesis pathways. Journal of Bacteriology 148, 64-71.
Oke, O.L. 1967 Chemical studies on the Nigerian food stuff "ogi". Food technology 21, 202-204.
Sands, D.C. & Hankin, L. 1974 Selecting lysine excreting mutants of lactobacilli for use in food and feed enrichment. Applied Microbiology 28, 523-524.
Sands, D.C. & Hankin, L. 1976 Fortification of foods by fermentation with lysine excreting mutants of lactobacilli. Journal of Agricultural and Food Chemistry 24, 1104-1106.
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Adebawo, O., Akingbala, J., Ruiz-Barba, J. et al. Utilization of high lysine-producing strains of Lactobacillus plantarum as starter culture for nutritional improvement of ogi. World Journal of Microbiology and Biotechnology 16, 451–455 (2000). https://doi.org/10.1023/A:1008967625335
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DOI: https://doi.org/10.1023/A:1008967625335