Abstract
Clostridium lentocellum SG6 fermented various pure crystalline cellulosic materials efficiently with maximum acetic acid yield (gram acetic acid/gram substrate) of 0.67, at low substrate (8 g l−1) concentration. The strain grew poorly on crude biopolymers but fermented them easily after alkali treatment, when grown with 8 g substrate l−1 concentration of alkali-extracted cotton straw (AECS), paddy straw (AEPS) and sorghum stover (AESS) etc. The acetic acid to substrate (A/S) ratios were similar to those obtained with pure cellulosic materials. An increase in substrate concentration led to a decreased A/S ratio and a decreased percentage of substrate degraded. At high substrate concentration of 75 g filter paper l−1, the strain SG6 converted 63.2 g filter paper into 31.28 g acetic acid l−1. At 100 g l−1 concentrations, AECS and AEPS served as the best substrates for acetic acid production when compared with other biopolymers. A maximum amount of 30.98 and 30.86 g acetic acid was produced from 70.6 g AEPS and 70.1 g AESS l−1 of medium by strain SG6, respectively. Acetic acid production of 0.67 g g−1 pure cellulose (Whatman No. 1 filter paper), 0.63 g g−1 of alkali-treated cotton straw (AECS) are the highest among the cellulolytic bacteria reported so far in mono culture fermentations with pure and native cellulosic materials.
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References
Anonymous, 1991 Biomass Generation and Utilization. Technology, Information, Forecasting and Assessment Council, pp. 1-185. New Delhi: Department of Science and Technology.
Beguin, P. & Aubert, J.P. 1994 The biological degradation of cellulose. FEMS Microbiology Reviews 13, 25-58.
Cheryan, M., Parekh, S., Shah, M. & Witjitra, K. 1997 Production of acetic acid by Clostridium thermoaceticum. Advances in Applied Microbiology 43, 1-33.
Datta, R. 1981 Acidogenic fermentation of corn stover. Biotechnology and Bioengineering 23, 61-77.
Donefer, E., Adeleye, I. & Jones, T. 1969 Effect of urea supplementation on the nutritive value of NaOH treated oat straw. Advances in Chemistry Series 95, 328-342.
Duong, T.V., Johnson, E.A. & Demain, A.L. 1983 Thermophilic anaerobic and cellulolytic bacteria. Topics in Enzyme Fermentation Biotechnology 7, 156-195.
Ebner, H., Sellmer, S. & Follmann, H. 1996 Acetic acid. In Biotechnology, products of primary metabolism, eds. Rehm, H.J. & Reed, G., vol. 6, pp. 381-401. Germany: VCH Verlagsgesells chaft MBH. ISBN 3-527-28316-1.
Fond, O., Petitdemanage, E., Petitdemange, H. & Engasser, J. 1983 Cellulose fermentation by a coculture of a mesophilic cellulolytic Clostridium and Clostridium acetobutylicum. Biotechnology and Bioengineering Symposium 13, 217-224.
Freier, D., Mothershed, C.P. & Wiegel, J. 1988 Characterization of Clostridium thermocellum JW 20. Applied and Environmental Microbiology 54, 204-211.
Ghose, T.K. & Bhadra, A. 1985 Acetic acid. In Comprehensive Biotechnology, ed. Moo-Young, M., vol. 3, pp. 701-729. Oxford: Pergamon Press, ISBN 0-08032511-4.
Gilbert, H.J. & Hazelwood, G.P. 1993 Bacterial cellulases and xylanases. Journal of General Microbiology 139, 187-194.
Khan, A.W., Asther, M. & Giuliano, C. 1984 Utilization of steam and explosion decomposed aspen wood by some anaerobes. Journal of Fermentation Technology 62, 335-339.
Kundu, S., Ghose, T.K. & Mukhopadhyay, S.N. 1983 Bioconversion of cellulose into ethanol by Clostridium thermocellum-production inhibition. Biotechnology Bioengineering 25, 1109-1125.
Lee, J. 1997 Biological conversion of lignocellulosic biomass to ethanol. Journal of Biotechnology 56, 1-24.
Levendis, Y.A. 1991 In Calcium Magnesium Acetate: An Emerging Bulk Chemical for Environmental Applications, eds. Wise, D., Levendis, Y. & Metghalchi, M., pp. 211-223. Amsterdam; Elsevier. ISBN 0-44488511-0.
Lowe, S.E., Jain, M.K. & Zeikus, J.G. 1993 Biology, ecology and biotechnological applications of anaerobic bacteria adapted to environmental stresses in temperature, pH, salinity or substrates. Microbiology Reviews 57, 451-509.
Lynd, L.R. 1989 Production of ethanol from lignocellulosic materials using thermophilic bacteria. Critical evaluation of potential and review. Advances in Biochemical Engineering and Biotechnology 38, 1-52.
Manivanan, S. & Wise, D. 1991 In Calcium Magnesium Acetate: An Emerging Bulk Chemical for Environmental Applications, eds. Wise, D., Levendis, Y. & Metghalchi, M., pp. 257-272. Amsterdam: Elsevier. ISBN 0-44488511-0.
Miller, T.L. & Wolin, M.J. 1995 Bioconversion of cellulose to acetate with pure cultures of Ruminococcus albus and a hydrogen-using acetogen. Applied and Environmental Microbiology 61, 3832-3835.
Parisi, F. 1989 Advances in lignocellulosics hydrolysis and in the utilization of the hydrolyzates. Advances in Biochemical Engineering and Biotechnology 38, 53-87.
Ravinder, T., Sudha Rani, K., Gopal Reddy & Seenayya, G. 1998 Direct conversion of biomass to acetic acid by anaerobic cellulolytic isolates. Journal of Scientific & Industrial Research 57, 591-594.
Rasmussen, M.A., Hespell, R.B., White, B.A. & Bothast, R.J. 1988 Inhibitory e.ect of methylcellulose on cellulose degradation by Ruminococcus flavifaciens. Applied and Environmental Microbiology 54, 890-897.
Ruyet, R.L., Dubourguirer, H.C. & Albagnac, G. 1984 Homoacetogenic fermentation of cellulose by coculture of Clostridium thermocellum and Acetogenium kivui. Applied and Environmental Microbiology 48, 893-894.
Sai Ram, M. & Seenayya, G. 1991 Production of ethanol from straw and bamboo pulp by primary isolates of Clostridium thermocellum. World Journal of Microbiology and Biotechnology 7, 372-378.
Sai Ram, M., Swamy, M.V. & Seenayya, G. 1991 Fermentation characteristics of ethanol Producing isolates of Clostridium thermocellum. Indian Journal of Microbiology 31, 175-180.
Sugaya, K., Tuse, D. & Jones, J.L. 1986 Production of acetic acid by Clostridium thermoaceticum in batch and continuous fermentations. Biotechnology and Bioengineering 28, 678-683.
Swamy, M.V. & Seenayya, G. 1996 Thermostable pullulanase and α-amylase activity from Clostridium thermosulfurogenes SV9–Optimization of culture conditions for enzyme production. Process Biochemistry 31, 157-162.
Visweswara Rao, K. 1996 A manual of statistical methods for use in Health, Nutrition and Anthropology. In Biostatistics pp. 237-284. New Delhi: Jaypee Brothers, Medical Publishers (P) limited.
Weimer, P.J. & Zeikus, J.G. 1977 Fermentation of cellulose and cellobiose by Clostridium thermocellum in the absence and presence of Methanobacterium thermoautotrophicum. Applied and Environmental Microbiology 33, 289-297.
Wise, D.L., Lavendis, Y.A. & Metgalchi, M. eds. 1991 Calcium Magnesium Acetate: An Emerging Bulk Chemical for Environmental Applications. Amsterdam: Elsevier Science Publishers. ISBN 0-44488511-0.
Wise, D.L. 1992 In Biochemical Engineering for 2001, eds. Furusaki, S., Endo, I. & Matsuno, R., pp. 723-726. Tokyo: Springer-Verlag. ISBN 4-43170091-9.
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Ravinder, T., Ramesh, B., Seenayya, G. et al. Fermentative production of acetic acid from various pure and natural cellulosic materials by Clostridium lentocellum SG6. World Journal of Microbiology and Biotechnology 16, 507–512 (2000). https://doi.org/10.1023/A:1008966205306
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DOI: https://doi.org/10.1023/A:1008966205306