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A rapid plate culture method for screening of amylase producing micro-organisms

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Biotechnology Techniques

Abstract

A simple and rapid method is described for the detection of amylase-producing micro-organisms on solid media using Remazol Brilliant Blue-starch as substrate. The blue starch is incorporated into nutrient agar and amylase production is detected by the disappearance of the colour around the colonies. The method which is non-destructive, allows for direct visualization and isolation of amylolytic micro-organisms from the environment without prior replication of colonies.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Agriculture, P.M.B. 2240, Abeokuta, Nigeria

    I. Akpan, M. O. Bankole & A. M. Adesemowo

Authors
  1. I. Akpan
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  2. M. O. Bankole
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  3. A. M. Adesemowo
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Akpan, I., Bankole, M.O. & Adesemowo, A.M. A rapid plate culture method for screening of amylase producing micro-organisms. Biotechnology Techniques 13, 411–413 (1999). https://doi.org/10.1023/A:1008965808641

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  • DOI: https://doi.org/10.1023/A:1008965808641

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