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Production and partial purification of naringinase by Penicillium decumbens PTCC 5248

World Journal of Microbiology and Biotechnology volume 16, pages 471–473 (2000)Cite this article

Abstract

Penicillium decumbens PTCC 5248 produced naringinase when grown in a medium contained naringin as a source of carbon. Rhamnose also induced production of naringinase. Prunin disappeared as the time of enzymatic reaction increased. On fractionation with isopropanol 24-fold purification was achieved. Optimum pH and temperature for naringinase activity were determined to be 4.5 and 55 °C respectively. The Km value of the enzyme with respect to naringin was found to be 1.7 mM. Citric acid, glucose, Ca2+, Mg2+, Zn2+ all inhibited naringinase activity.

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Authors and Affiliations

  1. Biotechnology Research Center, IROST, Tehran, Iran

    D. Norouzian, A. Hosseinzadeh, D. Nouri Inanlou & N. Moazami

Authors
  1. D. Norouzian
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  2. A. Hosseinzadeh
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  3. D. Nouri Inanlou
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  4. N. Moazami
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Norouzian, D., Hosseinzadeh, A., Inanlou, D.N. et al. Production and partial purification of naringinase by Penicillium decumbens PTCC 5248. World Journal of Microbiology and Biotechnology 16, 471–473 (2000). https://doi.org/10.1023/A:1008962131271

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  • DOI: https://doi.org/10.1023/A:1008962131271

  • Naringinase
  • Penicillium decumbens