Abstract
In vitro regeneration of wild groundnut species from Section Extranervosae (Arachis villosulicarpa, A. macedoi, A. retusa, A. burchellii, A. pietrarellii, A. prostrata, A. aff. prostrata and a new species) was examined for the purpose of germplasm renewal and conservation. Seeds of different ages, stored at the seed bank of CENARGEN/EMBRAPA were either inoculated on culture medium or used as a source of embryo axis and cotyledon explants. Whole seeds failed to germinate on MS either without growth regulators (MS0) or supplemented with 10 μM TDZ. Embryo axes cultured on MS0 produced only single plants. In the presence of 8.8 μM BAP these explants showed multi-shoot formation. Cotyledons cultured on MS supplemented with 110 μM BAP developed adventitious shoots through direct organogenesis. Plant regeneration was obtained from A. villosulicarpa, A. macedoi, A. retusa, A. burchellii and A. pietrarellii both from embryo axes and cotyledons. Explants from A. prostrata and A. aff. prostrata did not produce regenerants. Rooting of shoots was induced in the presence of 5.4 μM NAA. Primary plants derived from these explants were further multiplied by culturing nodal segments on MS medium plus 2.7 μM NAA.
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References
Bajaj YPS, Kumar P, Labana KS and Singh MM (1981) Regeneration of plants from seedling explants & callus cultures of Arachis hypogaea L. Indian J. Exp. Biol. 19: 1026–1029
Benson E (1990) Free Radical Damage in Stored Plant Germplasm. IBPGR, Rome.
Beyer E (1976) Silver ion: a potent antiethylene agent in cucumber and tomato. HortSci. 11: 195–196
Buchvarov P and Gantcheff TS (1984) Influence of accelerated and natural ageing on free radical levels in soybean seeds. Physiol. Plant. 60: 53–56
Daimon H, Mu M (1991) Multiple shoot formation and plantlet regeneration from cotyledonary node in peanut (Arachis hypogaea L.). Japan J. Breed. 41: 461–466
Dunbar KB, Pittman RN and Morris JB (1993) In vitro culture of embryonic axes from Arachis species for germplasm recovery. J. Seed Technol. 17: 1–8
Engelmann F (1997) In vitro conservation research activities at the International Plant Genetic Resources Institute (IPGRI). Plant Tiss. Cult. Biotechnol. 3: 46–52
Freitas VG, Lacorte C, Sachetto-Martins G, Krul WR, Oliveira DE, Neves LJ and Mansur E (1997) Identification of competent cells for Agrobacterium-transformation and in vitro regeneration in peanut leaf and cotyledon explants. Braz. J. Plant Physiol. 9: 157–167
Gill R and Saxena PK (1992) Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): promotive role of thidiazuron. Canadian Journal of Botany 70: 1186–1192
Goh CJ, Ng SK, Lakshmanan P and Loh CS (1997) The role of ethylene on direct shoot bud regeneration from mangosteen (Gaecinia mangostana L.) leaves cultured in vitro. Plant. Sci. 124: 193–202
Hisajima S, Paek KY, Nanwongpron K, Subhadrabandhu S and Ishizuka K (1989) Mass propagation of peanut (Arachis hypogaea) plant cultured seeds in vitro. Japan J. Trop. Agr. 33: 237–242
Kanyand M, Dessai AP and Prakash CS (1994) Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogaea) plants in vitro. Plant. Cell Rep. 14: 1–5.
Mansur E, Lacorte C, Rabello ACG and Cordeiro AR (1993) In vitro regeneration of Arachis villosulicarpa Hoehne from cotyledon segments, leaves and cell suspension. Pesq. Agropec. Bras. 28: 1143–1146
McKently AH, Moore GA and Gardner FP (1990) In vitro plant regeneration of peanut from seed explants. Crop Sci. 30: 192–196
Morris JB, Dunn S and Pittman RN (1995) Plant recovery from embryonic axes of deteriorated peanut seed for germplasm renewal. Peanut Sci. 22: 66–70
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Planta. 15: 473–497
Murthy BNS, Murch SJ and Saxena PK (1995) Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): Endogenous growth regulator levels and significance of cotyledons. Physiol. Planta. 94: 268–276
Pestana MC, Lacorte C, Freitas VG and Oliveira DE and Mansur E (1999) In vitro regeneration of peanut (Arachis hypogaea L.) through organogenesis: effect of culture temperature and AgNO3. In vitro Cell Dev. Biol. Plant. 35: 214–216
Pittman RN, Johnson BB and Banks DJ (1984) In vitro differentiation of a wild peanut, Arachis villosulicarpa Hoehne. Peanut Sci. 11: 24–27
Powell AA and Matthews S (1978) The damaging effect of water on dry pea embryos during imbibition. J. Exp. Bot. 29: 1215–1229
Rani AS and Reddy GM (1996) Multiple shoot regeneration from de-embryonated cotyledons of cultivated and wild species of Arachis. J. Gen. Breed. 50: 351–355
Roberts EH and Ellis RH (1989) Water and Seed Survival. Ann. Bot. 63: 39–52
Roos EE (1980) Physiological, biochemical, and genetic changes in seed quality during storage. Hortsci. 15: 781–784
Still PE, Plata MI, Campbell RJ, Bueno LC, Chichester EA and Niblett CL (1987) Regeneration of fertile Arachis paraguariensis plants from callus and suspension cultures. Plant Cell, Tiss. Org. Cult. 9: 37–43
Valls JFM, Rao VR, Simpson CE and Krapovickas A (1985) Current status of collection and conservation of South American groundnut germplasm with emphasis on wild species of Arachis. Proceedings of an International Workshop on Cytogenetics of Arachis, 31 Oct–2 Nov 1983, ICRISAT Center, India. Patancheru, A.P. 502 324, India: ICRISAT, 15–35
Vàzquez-Yanes C and Arèchiga MR (1996) Ex situ conservation of tropical rain forest seed: problems and perspectives. Interciencia 21: 293–298
Venkatachalam P and Jayabalan N (1997) Effect of auxins and cytokinins on efficient plant regeneration and multiple-shoot formation from cotyledons and cotiledonary-node explants of groundnut (Arachis hypogaea L.) by in vitro culture technology. Appl. Biochem. Biotech. 67: 237–247
Venkatachalam P, Geetha N, Khandelwal A, Shaila MS and Sita GL (1999) Induction of direct somatic embryogenesis and plant regeneration from mature cotyledon explants of (Arachis hypogaea L.). Curr Sci. 77: 269–273
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Gagliardi, R., Pacheco, G., Coculilo, S. et al. In vitro plant regeneration from seed explants of wild groundnut species (Genus Arachis, Section Extranervosae). Biodiversity and Conservation 9, 943–951 (2000). https://doi.org/10.1023/A:1008960608543
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DOI: https://doi.org/10.1023/A:1008960608543