Abstract
The human rotavirus titer was optimal at an infection cell density of about 4–8 × 104 cells cm−2 in monkey kidney cell cultures. The highest viral titers (3.8 × 107 TCID50 ml−1 and 3.7 × 107 TCID50 ml−1) were obtained at an multiplicity of infection of 0.05 in well plate and T-flask, respectively.
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Chang, K.H., Park, CH., Park, J.H. et al. Optimal cell density and multiplicity of infection for the propagation of human rotavirus in monkey kidney cells. Biotechnology Techniques 13, 757–759 (1999). https://doi.org/10.1023/A:1008949726629
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DOI: https://doi.org/10.1023/A:1008949726629