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Transference of a crystal protein gene from Bacillus thuringiensis and its expression in Bradyrhizobium sp. cells

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Abstract

The plasmid pHT409 that harbours the cryIA(a) gene for the production of a δ-endotoxin (crystal protein) from Bacillus thuringiensis was transferred into Bradyrhizobium sp. A conjugal transfer system aiming to introduce the plasmid into the Bradyrhizobium sp. host from colonies of an Escherichia coli donor strain (DH5α::pHT409) has been developed. As a result exconjugants were obtained in which the transferred plasmid has been detected by both microbiological and electrophoresis techniques. The cryIA(a) gene when inside the new host had a low expression level which was detected by immunoblotting.

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References

  • Arantes, O., Menou, G., Sanchis, V., Azevedo, J.L. & Lereclus, D. 1990 Cloning of a Bacillus thuringiensis crystal protein gene. Revista Brasileira de Genética 13, 654-652.

    Google Scholar 

  • Beringer, J.E., Beynon, J.L., Buchanan-Wollaston, A.V. & Johnston, A.W.B. 1978 Transfer of the resistance transposon Tn-5 to Rhizobium. Nature 276, 633-634.

    Google Scholar 

  • Donovan, W.P., Gonzales, J.M., Gilbert, P. & Dankocsik, C. 1988 Isolation and characterization of EG2158, a new strain of Bacillus thuringiensis toxic to coleopteran larvae, and nucleotide sequence of the toxin gene. Molecular & General Genetics 214, 365-372.

    Google Scholar 

  • Goze, A. & Ehrlich, S.D. 1980 Replication of plasmids from Staphylococcus aureus in Escherichia coli. Proceedings of the National Academy of Sciences 77, 7333-7337.

    Google Scholar 

  • Guerinot, M.L. & Chelm, B.K. 1986 Bacterial δ-aminolevulinic acid synthase activity is not essential for leghemoglobin formation in the soybean/Bradyrhizobium japonicum symbiosis. Proceedings of the National Academy of Sciences 83, 1837-1841.

    Google Scholar 

  • Harlow, E. & Lane, D. 1988 Antibodies: A Laboratory Manual. pp 471-510. Cold Spring Harbor Laboratory Press, ISBN 0-87969314-2.

  • Hattermann, D.R. & Stacey, G. 1990 Efficient DNA Transformation of Bradyrhizobium japonicum by Electroporation. Applied and Environmental Microbiology 56, 833-836.

    Google Scholar 

  • Lereclus, D., Arantes, O., Chauffaux, J. & Lecadet, M.M. 1989 Transformation and expression of a cloned δ-endotoxin gene in Bacillus thuringiensis. FEMS Microbiology Letters 60, 211-218.

    Google Scholar 

  • Lima, P.S.C., Sena, J.A.D., Silva, J.R. & Lemos, M.V.F. Plasmid pHT409 introduction by electrotransformation of Escherichia coli DH5α strain. In press (Cientifica).

  • Mazodier, P. & Davies, J. 1991 Gene transfer between distantly related bacteria. Annual Review of Genetics 25, 147-171.

    Google Scholar 

  • Nambiar, P.T.C., Ma, S.W. & Iyer, V.N. 1990 Limiting an insect infestation of nitrogen-®xing root nodules of the pigeon pea (Cajanus cajan) by engineering the expression of an entomocidal gene in its root nodules. Applied and Environmental Microbiology 56, 2866-2869.

    Google Scholar 

  • Sambrook, J., Fritsch, E.F. & Maniatis, T. 1989 Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, New York. ISBN 0-87969309-6.

    Google Scholar 

  • Sanchis, V., Lereclus, D., Menou, G., Chanfaux, J. & Lecadet, M.M. 1988. Multiplicity of δ-endotoxin genes with di.erent insecticidal specificities in Bacillus thuringiensis aizawai 7-29. Molecular Microbiology 2, 393-404.

    Google Scholar 

  • Schäfer, A., Kalinowsli, J., Simon, R., Seep-Feldhaus, A. & Püller, A. 1990 High-frequency conjugal plasmid transfer from Gram-negative Escherichia coli to various Gram-positive coryneiform bacteria. Journal of Bacteriology 172, 1663-1666.

    Google Scholar 

  • So, J.S., Hodgson, A.L.M., Haugland, R., Leavitt, M., Banfalvi, Z., Niewhoop, H.J. & Stacey, G. 1987 Transposon-induced symbiotic mutants of Bradyrhizobium japonicum: Isolation of two gene regions essential for nodulation. Molecular & General Genetics 207, 15-23.

    Google Scholar 

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Authors and Affiliations

  1. EMBRAPA/CPAMN, Av. Duque de Caxias, 5650, 64056-220, Teresina, Brasil

    Paulo Sarmanho da Costa Lima

  2. Depto. de Biologia Aplicada à Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, UNESP, Via Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, SP, Brasil

    Manoel Victor Franco Lemos

  3. Depto. de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP, 14870-000, Jaboticabal, SP, Brasil

    Eliana G. Macedo Lemos & Lúcia Maria Carareto Alves

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  1. Paulo Sarmanho da Costa Lima
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  2. Manoel Victor Franco Lemos
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  3. Eliana G. Macedo Lemos
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  4. Lúcia Maria Carareto Alves
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da Costa Lima, P.S., Lemos, M.V.F., Lemos, E.G.M. et al. Transference of a crystal protein gene from Bacillus thuringiensis and its expression in Bradyrhizobium sp. cells. World Journal of Microbiology and Biotechnology 16, 361–365 (2000). https://doi.org/10.1023/A:1008948125429

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