Abstract
Reverse micelles serve as a novel tool to entrap enzymes and microbial whole cells within aqueous pockets and can be of great use in enhancing the efficiency and sustainability of the biological system. Photosynthetic bacterium Rhodopseudomonas sphaeroides entrapped inside the aqueous pool of reverse micelles prepared from benzene-sodium lauryl sulphate exhibited 25-fold enhancement of H2 photoproduction rate (1.67 ml H2 [mg protein]−1 h−1) compared to cells suspended in normal aqueous medium. Hydrogen photoproduction by the bacterium was catalysed by the nitrogenase enzyme system which was supported at a low light intensity of 12 μEm−2 sec−1 photon flux energy at a wavelength of 520 nm. The optimum temperature for the process was 40 °C.
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References
Fibler, J.K., Kohring, G.W. & Giffhorn, F. 1995 Enhanced hydrogen production from aromatic acids by immobilized cells of Rhodopseudomonas palustris. Applied Microbiology and Biotechnology 44, 43–46.
Gajjar, L., Singh, A., Dubey, R.S. & Srivastava, R.C. 1997 Enzymic activity of the whole cells entrapped into reversed micelles - Studies on alpha amylase and invertase in the entrapped yeast cells. Applied Biochemistry and Biotechnology 66, 159–172.
Hartel, U. & Buckel, W. 1996 Sodium ion dependent hydrogen production in Acidaminococcus fermentans. Archives of Microbiology 166, 350–356.
Kashyap, A.K., Pandey, K.D. & Sarkar S. 1995 Enhanced hydrogen photoproduction by non-heterocystous cyanobacterium plectonema boryanum. International Journal of Hydrogen Energy 21, 107–109.
Miyake, J., Mao, X.Y. & Kawamura, S. 1985 Hydrogen production using an immobilized co-culture of anaerobic and photosynthetic bacteria. Proceedings of the International Symposium on Hydrogen Systems, Vol. I, Beijing, China, pp. 277–281.
Miyake, J. & Kawamura, S. 1987 Effciency of light energy conversion to hydrogen by the photosynthetic bacterium Rhodobacter sphaeroides. International Journal of Hydrogen Energy 12, 147–149.
Mitsui, A., Matsunaga, T., Ikemoto, H. & Renuka, B.R. 1985 Organic and inorganic waste treatment and simultaneous photoproduction of hydrogen by immobilized photosynthetic bacteria. Developments in Industrial Microbiology 26, 209.
Pfennig, N. 1967 Photosynthetic bacteria. Annual Review of Microbiology 21, 285–324.
Singh, S.P., Srivastava, S.C. & Pandey, K.D. 1990 Photoproduction of hydrogen by a non-sulphur bacterium isolated from root zones of water fern Azolla pinnata. International Journal of Hydrogen Energy 15, 403–406.
Singh, S.P., Srivastava, S.C. & Pandey, K.D. 1993 Hydrogen production by Rhodopseudomonas at the expense of vegetable starch, sugarcane juice and whey. International Journal of Hydrogen Energy 19, 437–440.
Sasikala, Ch., Ramana, Ch. V. & Prasad, G.S. 1994 H2 production by mixed cultures. World Journal of Microbiology and Biotechnology 10, 221–223.
Vicenzini, M., Materassi, R., Tredici, M.R. & Florenzano, G. 1982 Hydrogen production by immobilized cells. II. H2 photoevolution and waste water treatment by agar entrapped cells of Rhodopseudomonas palustris and Rhodospirillum malishianum. International Journal of Hydrogen Energy 9, 725–728.
Xu, X., Yu, X., Zheng, P., Chen, W. & Feng, X. 1995 Studies on kinetics of substrate utilization of hydrogen production from waste water with immobilized cells of photosynthetic bacteria. Chinese Journal of Biotechnology 11, 67–77.
Zurrer, H. & Bachofen, R. 1979 Hydrogen production by the photosynthetic bacterium of Rhodospirillum rubrum. Applied and Environmental Microbiology 37, 789–793.
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Singh, A., Pandey, K. & Dubey, R. Reverse micelles: a novel tool for H2 production. World Journal of Microbiology and Biotechnology 15, 277–282 (1999). https://doi.org/10.1023/A:1008942928804
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DOI: https://doi.org/10.1023/A:1008942928804