Abstract
A simple and convenient system for quantitatively measuring the number of adsorbed animal cells per unit of bubble surface area (Γ, unit: cells/cm2) was developed. The system was successfully applied to recombinant Chinese hamster ovary (r-CHO) suspension cultures to investigate the dynamic cell-bubble attachment in a bubble column. In serum-free medium, Γ values increased with bubble rising height (H) and cell concentration (C) and then became constant (about 1750 cells/cm2) when H and C were sufficiently high. In medium containing protective additives, the trends of Γ values with H were similar to that in serum-free medium. Compared with serum-free medium, polyvinyl-pyrrolidone (PVP) increased the Γ values to 1941 cell/cm2 whereas other tested additives decreased the values of Γ in some different degree.
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Wen, ZY., Tan, WS. Quantitative measurement of cell-bubble attachment in bubble columns using foam flotation techniques. Biotechnology Techniques 13, 131–135 (1999). https://doi.org/10.1023/A:1008923005392
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DOI: https://doi.org/10.1023/A:1008923005392