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Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii

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Biotechnology Techniques

Abstract

Pectin releasing activity of protopectinase SE (PPase-SE) from Geotrichum klebahnii (= G. penicillatum = Trichosporon penicillatum) ATCC 42397 was determined using different batches of lemon protopectin as substrate. Results obtained showed a high degree of variability depending on the batch of protopectin used. As PPase-SE also shows polygalacturonase (PGase) activity, a method for the assay of this activity was optimised. The best assay conditions were: substrate (polygalacturonic acid) concentration of 2.0 g 1−1, reaction time of 10 min and up to 0.17 PGase units per test tube.

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Authors and Affiliations

  1. Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI), CONICET-UNLP, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, 1900, La Plata, Argentina

    Sebastián F. Cavalitto, Roque A. Hours & Carlos F. Mignone

Authors
  1. Sebastián F. Cavalitto
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  2. Roque A. Hours
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  3. Carlos F. Mignone
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Cavalitto, S.F., Hours, R.A. & Mignone, C.F. Quantification of protopectinase SE, an endopolygalacturonase with pectin-releasing activity from Geotrichum klebahnii . Biotechnology Techniques 13, 385–390 (1999). https://doi.org/10.1023/A:1008917912296

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  • DOI: https://doi.org/10.1023/A:1008917912296

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