Abstract
Comprehensive analyses of spatially organized microbial systems require the use of techniques which can investigate microorganisms in defined regions of space. In this work, an in-situ image analysis method was developed which allowed the quantitative description of Lactobacillus plantarum biomass levels at different locations within alginate beads. Using this technique the kinetic properties of the immobilised biomass were determined in 50 μm increments across the radius of the alginate bead.
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Condron, P., McLoughlin, A.J. & Upton, M. Quantitative determination of the spatial distribution of microbial growth kinetics within alginate beads using an image analysis technique. Biotechnology Techniques 13, 927–930 (1999). https://doi.org/10.1023/A:1008914429652
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DOI: https://doi.org/10.1023/A:1008914429652