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Development of enzyme linked immunosorbent assays to measure Bm86 antigen of Boophilus microplus (cattle tick) and to detect anti-Bm86 antibodies in serum samples

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Biotechnology Techniques

Abstract

The immunization of cattle with the Boophilus microplus Bm86 antigen has been successful for the control of cattle tick infestations. To monitor the Bm86 production process and to measure the anti-Bm86 antibody titers in vaccinated cattle, mAb-based ELISA were developed and validated. The development of both immunological methods is essential to obtain a product with high quality and immunogenic properties and to monitor the immunological protection induced in vaccinated cattle against B. microplus.

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References

  • Canales M, Enríque A, Ramos E, Cabrera D, Dandie H, Soto A, Falcón V, Rodríguez M, de la Fuente J (1997) Vaccine 15: 414-422.

    Google Scholar 

  • de la Fuente J, Rodríguez M, Redondo M, Montero C, García García JC, Mendez L, Serrano E, Valdes M, Enrique A, Canales M, Ramos E, Boue O, Machado H, Lleonart R, de Armas CA, Rey S, Rodriguez JL, Artiles M, Garcia L (1998) Vaccine 16: 366-373.

    Google Scholar 

  • Enríquez A, Canales M, Ramos E, Dandie H, Boué O, Soto A, Cabrera D (1995) Production of a recombinant vaccine against Boophilus microplus. In: de la Fuente J, ed. Recombinant Vaccines for the Control of Cattle Tick. Havana: Elfos Scientiae, pp. 99-102.

    Google Scholar 

  • Enríquez A, Boué O, Redondo M, Montero, C, Machado H, Joglar M, Soto A, Rodríguez, M, de la Fuente J (1999) Biotechnol. Appl. 16: 15-17.

    Google Scholar 

  • Fazekas de St. Groth S, Scheidegger D (1980) J. Immunol. Methods 35: 1-3.

    Google Scholar 

  • Horlow E, Lanem D (1989) In: Horlow E, Lane D, eds. Antibodies, a Laboratory Manual. New York: Cold Spring Harbor Laboratory, p. 471.

    Google Scholar 

  • Kemp DH, Willadsen P (1991) In: Cruz V, Fragoso H, García V, Quiroz H, Solis S, Torres B, Vega C, eds. Segundo Seminario Internacional de Parasitología Animal. Morelos Mexico: Oaxtepec, pp. 45-56.

    Google Scholar 

  • Kyhse-Anderson J (1984) J. Biochem. Biophys. Methods 10: 203-209.

    Google Scholar 

  • Lane RD (1985) J. Immunol. Methods 81: 223.

    Google Scholar 

  • Lowry DH, Resembrough NJ, Farr AL, Randall RK (1951) J. Biol. Chem. 193: 265-269.

    Google Scholar 

  • Nolan J, Wilson JT, Green PE, Bird PE (1989) Aust. Vet. J. 66: 179-182.

    Google Scholar 

  • Ouchterlouy O, Nielson L (1978) In: Weir DM, ed. Handbook of Experimental Immunology. Oxford: Blackwell Scientific Publications, pp. 559-566.

    Google Scholar 

  • Rand KN, Moore T, Sriskantha A, Spring K, Tellam R, Willadsen P, Cobon G (1989) Proc. Natl. Acad. Sci. USA 86: 9657-9661.

    Google Scholar 

  • Redondo M, Fragoso H, Ortíz M, Montero C, Lona J, Medellín JA, Fría R, Hernández V, Franco R, Machado H, Rodríguez M, de la Fuente J (1999) J. Exp. Appl. Acarol. (submitted).

  • Rodríguez M, Rubiera R, Penichet M, Montesino R, Cremata J, Falcón V, Sánchez G, Bringas R, Cordovés C, Valdés M, Lleonart R, Herrera L, de la Fuente J (1994) J. Biotechnol. 33: 135-146.

    Google Scholar 

  • Rodríguez M, Penichet M, Mourís AE, Labarta V, Lorenzo-Luaces L, Rubiera R, Cordovés C, Sánchez PA, Ramos E, Soto A, Canales M, Palenzuela D, Triguero A, Lleonart R, Herrera L, de la Fuente J (1995a) Vet. Parasitol. 57: 339-349.

    Google Scholar 

  • Rodríguez M, Massard CL, Da Fonseca AH, Ramos FN, Machado H, Labarta V, de la Fuente J (1995b) Vaccine 13: 1804-1808.

    Google Scholar 

  • Rodríguez M, Montero C, Labarta V, de la Fuente J (1995c) Effect of vaccination with Gavac on the incidence of Bebesia bovis infections and the reduction in the number and frequency of acaricide treatments in cattle under production conditions in Cuba. In: de la Fuente J, ed. Recombinant Vaccines for the Control of Cattle Tick. Havana: Elfos Scientiae, pp. 187-194.

    Google Scholar 

  • Tellam RL, Smit D, Kemp DH, Willadsen P (1992) Vaccination against tick. In: Yong WK, ed. Animal Parasite Control Utilizing Biotechnology. Boca Raton: CRC Press, pp. 303-331.

    Google Scholar 

  • Wikel SK (1988) Vet. Parasitol 29: 235-264.

    Google Scholar 

  • Willadsen P, Kemp DH (1988) Parasitology Today 4: 196-198.

    Google Scholar 

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Authors and Affiliations

  1. Centro de Ingeniería Genética y Biotecnología, P.O.Box 83, Sancti Spiritus, Cuba

    A. Triguero & R. Blanco

  2. Mammalian Cell Genetics Division. Centro de Ingeniería Genética y Biotecnología, P.O. Box 6162, Havana, Cuba

    H. Machado, M. Rodríguez & J. de la Fuente

Authors
  1. A. Triguero
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  2. R. Blanco
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  3. H. Machado
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  4. M. Rodríguez
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  5. J. de la Fuente
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Triguero, A., Blanco, R., Machado, H. et al. Development of enzyme linked immunosorbent assays to measure Bm86 antigen of Boophilus microplus (cattle tick) and to detect anti-Bm86 antibodies in serum samples. Biotechnology Techniques 13, 119–125 (1999). https://doi.org/10.1023/A:1008906003575

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  • DOI: https://doi.org/10.1023/A:1008906003575

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