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Characterisation of percentage viability of Streptomyces clavuligerus using image analysis

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Biotechnology Techniques

Abstract

A staining procedure has been developed to identify the different physiological states of Streptomyces clavuligerus using the fluorescent, bacterial viability stain BacLight. An image analysis method was used to quantify the extent of the differently stained regions, and therefore to measure the percentage viability. In a batch fermentation, the viability of the mycelia decreased to 64% during the rapid growth phase. Following a period of fragmentation, the viability was approximately constant at 75% until late in the fermentation, when it increased to 93%.

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Authors and Affiliations

  1. Centre for Bioprocess Engineering, School of Chemical Engineering, The University of Birmingham, Birmingham, B15 2TT, UK

    I. M. Sebastine, S. M. Stocks, P. W. Cox & C. R. Thomas

Authors
  1. I. M. Sebastine
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  2. S. M. Stocks
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  3. P. W. Cox
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  4. C. R. Thomas
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Sebastine, I.M., Stocks, S.M., Cox, P.W. et al. Characterisation of percentage viability of Streptomyces clavuligerus using image analysis. Biotechnology Techniques 13, 419–423 (1999). https://doi.org/10.1023/A:1008902330043

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  • DOI: https://doi.org/10.1023/A:1008902330043

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