Abstract
A new micromanipulation technique which has previously been used to measure the mechanical properties of single animal cells has now been applied to yeast cells. In this study this technique was used to measure yeast cell strength and cell size across a 2l batch fermentation. Alternatively the cell size could also be determined using a Coulter counter while cell measurement was diluted with a conducting fluid (Isoton II). For the cell strength, it was found that the osmotic pressure of diluents did affect cell strength. However, it was also found that there was no significant effect of osmotic pressure of diluents on cell size whether a Coulter counter or micromanipulation was used for measurement. Micromanipulation has been shown to be a powerful technique for measuring the mechanical properties of yeast cells and it will be very useful for studying their behaviour in cell disruption equipment, e.g. high-pressure homogenizers.
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Srinorakutara, T. Study on effect of diluent osmotic pressure on yeast cell strength and cell size using a novel micromanipulation technique. World Journal of Microbiology and Biotechnology 14, 719–725 (1998). https://doi.org/10.1023/A:1008892116065
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DOI: https://doi.org/10.1023/A:1008892116065