Abstract
The use of a dissolvable solid matrix, κ-carrageenan, to quantify biomass grown on solid media was studied. A firm gel was obtained with 2% (w/v) κ-carrageenan and 20 mM K+ which could be easily dissolved in demineralized water. Direct quantification of Coniothyrium minitans biomass grown on this medium was feasible. No effects of the dissolution on the amount of biomass recovered were detected.
Similar content being viewed by others
References
Barrios-Gonzáles J, Mejía A (1996) Biotechnol. Ann. Rev. 2: 85-121.
Desgranges C, Vergoignan C, Georges M, Durand A (1991) Appl. Microbiol. Biotechnol. 35: 200-205.
Garraway MO, Evans RC (1984) Inorganic nutrients. In: Fungal Nutrition & Physiology. New York: John Wiley & Sons, pp. 124-170.
Guiseley KB (1989) Enzyme Microbiol. Technol. 11: 706-716.
Hanson RS, Phillips JA (1981) Chemical composition. In: Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Phillips GB, eds. Manual of Methods for General Bacteriology. Washington DC: American Society for Microbiology, pp. 328-364.
McQuilken MP, Budge SP, Whipps JM (1997) Biocontrol Sci. Technol. 7: 23-36.
Mitchell DA (1992) Biomass determination in solid-state cultivation. In: Doelle HW, Mitchell DA, Rolz CE, eds. Solid Substrate Cultivation. London: Elsevier Science Publishers, pp. 53-63.
Mitchell DA, Greenfield PF, Doelle HW (1986) Biotechnol. Lett. 8: 827-832.
Mudgett RE (1986) Solid-state fermentations. In: Demain AL, Solomon NA, eds. Manual of Industrial Microbiology and Biotechnology. Washington DC: American Society for Microbiology, pp. 66-83.
Ooijkaas LP, Tramper J, Buitelaar RM (1998) Enzyme Microbiol. Technol. 22: 480-486.
Reeslev M, Kjøller A (1996) Appl. Environ. Microbiol. 61: 4236-4239.
Roels JA (1983) Energetics and Kinetics in Biotechnology. Amsterdam: Elsevier Biomedical Press.
Tosa T, Sato T, Mori T, Yamamoto K, Takata I, Nishida Y, Chibata I (1979) Biotechnol. Bioeng. 21: 1697-1709.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Weber, F.J., Tramper, J. & Rinzema, A. Quantitative recovery of fungal biomass grown on solid κ- carrageenan media. Biotechnology Techniques 13, 55–58 (1999). https://doi.org/10.1023/A:1008891614920
Issue Date:
DOI: https://doi.org/10.1023/A:1008891614920