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Gene transfer in silkworm Bombyx mori via electroporation

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Biotechnology Techniques

Abstract

Electroporation conditions have been standardized for the mass mobilization of foreign DNA into silkworm eggs. The hatchability of egg varied depending upon the electroporatic conditions. The maximum hatchability of 68% was at the electroporatic condition of 200V/cm and 25μF with two pulses. Electroporation has resulted in successful transfer of foreign DNA into silkworm eggs and it has been confirmed by slot blot, Southern blot and PCR analysis. © Rapid Science Ltd. 1998

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Authors
  1. Y. Shamila
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  2. S. Mathavan
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Shamila, Y., Mathavan, S. Gene transfer in silkworm Bombyx mori via electroporation. Biotechnology Techniques 12, 507–510 (1998). https://doi.org/10.1023/A:1008891111905

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  • DOI: https://doi.org/10.1023/A:1008891111905

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