Abstract
Titers of human rotavirus, grown in T-flasks, were optimal in the medium with no serum, 30 μg DEAE-dextran/ml or 0.5% (v/v) dimethyl sulfoxide. Cholesterol (0.34 mM) enhanced the viral titer. Supplementation with all three additives increased the titer to 5.62 x 106 TCID50 (50% tissue culture infectious dose)/ml, 3.2 times the level of the control culture. Using the same supplemented medium in a split-flow air-lift bioreactor, the highest titer (3.16 x 106 TCID50/ml) was obtained at an air/CO2 (95:5, v/v) flow rate of 30 ml/min. The split-flow air-lift bioreactor proved to be suitable for rotavirus propagation from rhesus monkey kidney cells.
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Birch, C.J., Rodger, S.M., Marshall, J.A. and Gust, I.D. (1983). J. Med. Virol., 11, 241-250.
Brodelius, P. and Nilsson, K. (1983). Applied Microbiol. Biotechnol., 17, 275-280.
Chung, I.S., Taticek, R. and Shuler, M.L. (1993). Biotechnol Prog., 9, 675-678.
Griffiths, B. and Looby, D. (1991). Fixed and immobilized beads for the cultivation of animal cells. In: Animal Cell Bioreactors, C.S. Ho and D.I.C. Wang, eds. pp165-166, Madison: Butterworth-Heinemann.
Gor, D. and Lucassen, E. (1994). Characterization of cell lines. In: In-vitro Cultivation of Animal Cells, C.K. Leach, ed. pp92-93, Oxford: Butterworth-Heinemann.
Hasegawa, A.S., Matsuno, S., Inouye, S., Kono, R., Tsurukubo, Y., Mukoyama, A. and Saito, Y. (1982). J. Clin. Microbiol., 16, 387-390.
Kaplan, M.M., Wiktor, J.J., Maes, R.F., Campbell, J.B. and Koprowski, H. (1967). J. of Virol., 1, 145-151.
Konno, T., Suzuki, H., Kitaoka, S., Sato, T., Fukuhara, N., Yoshie, O., Fukudome, K. and Numazaki, Y. (1993). Clin. Infectious Diseases, 16, S92-97.
Ramirez, O.T. and Mutharasan, R. (1990). Biotechnol. Bioeng., 36, 911-920.
Reed, L.F. and Muench, H. (1938). Am. J. Hyg., 27, 493-497.
Sato, K., Inaba, Y., Shinozaki, T., Fujii, R. and Matsmoto, M. (1981). Arch. Virol., 69, 155-160.
Suzuki, H., Kitaoka, S., Konno, T., Sato, T. and Ishida, N. (1985). Arch. Virol., 85, 25-34.
Urasawa, T., Urasawa, S. and Taniguchi, K. (1981). Microbiol. Immunol., 25, 1025-1035.
Ward, R.L., Knowlton, D.R. and Pierce, M.J. (1984). J. Clin. Microbiol., 19, 748-753.
Wyatt, R.G., Kalica, A.R., Mebus, C.A., Kim, H.W., London, W.T., Chanock, R.M., Kapikian, A.Z. (1978). Reovirus-like agents (rotaviruses) associated with diarrheal illness in animals and mans. In: Perspectives in Virology, M. Pollard, ed. vol 10, New York: Raven Press.
Wyatt, R.G., James, W.D., Bohl, E.H., Theil, K.W., Saif, L.H., Kalica, A.R., Greenburg, H.R., Kapikian, A.Z. and Chanock, R.M. (1980). Science, 207, 189-191.
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Park, J.H., Lee, K.W. & Chung, I.S. Improved propagation of human rotavirus from cell cultures of rhesus monkey kidney cells using medium supplemented with DEAE-dextran, dimethyl sulfoxide and cholesterol. Biotechnology Techniques 12, 7–10 (1998). https://doi.org/10.1023/A:1008886905753
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DOI: https://doi.org/10.1023/A:1008886905753