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Effect of carbon source on the biotransformation enzymesin Serratia marcescens

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Abstract

A microorganism capable of degrading camphor as the sole source of carbon was isolated from soil. The strain was identified as Serratia marcescens (NCIM 5115). The strain when grown in the peptone–glucose medium showed a doubling time of 2.7 h. This microorganism showed the presence of cytochrome P-450, cytochrome b5 and the activities of cytochrome c reductase, dichlorophenol indophenol reductase, aminopyrine-N-demethylase and steroid 11-β-hydroxylase. A significant increase in all activities was observed when cells were incubated for 3h in a medium containing either 0.2% camphor, 1.0% n-hexadecane or 0.1% naphthalene when compared to the peptone–glucose medium.

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Authors and Affiliations

  1. Department of Biochemistry, Shivaji University, Kolhapur –, 416 004, India

    M.D. Salokhe & S.P. Govindwar

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  1. M.D. Salokhe
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  2. S.P. Govindwar
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Salokhe, M., Govindwar, S. Effect of carbon source on the biotransformation enzymesin Serratia marcescens. World Journal of Microbiology and Biotechnology 15, 259–263 (1999). https://doi.org/10.1023/A:1008875404889

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