Abstract
The effect of substratum roughness on the adhesion of Pseudomonas aeruginosa and Staphylococcus epidermidis was investigated using PMMA. A small increase in Ra values (0.04–1.24 μm) resulted in a significant increase (P<0.05) in bacterial attachment. Subsequent increases in surface roughness (Ra=1.86–7.89 μm) resulted in a decrease in adhesion, although adhesion was still higher than to the smooth surface. When the PMMA surfaces were coated with protein (bovine serum albumin), no difference (P<0.05) could be determined in the amount of protein adsorbed, irrespective of surface topography. However, the influence of the underlying topography on adhesion was still evident. Substratum topography is an important parameter affecting bacterial adhesion to surfaces.
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References
E. W. Allister, L. C. Carey, P. G. Brady, R. Heller and S. G. Kovacs, Gastrointest. Endosc. 39 (1993) 422.
W. A. Sammons, J. Br. Contact Lens Assoc. 8 (1) (1985) 31.
W. A. Muller, L. H. Cohn and F. J. Schoen, Am. J. Cardiol. 54 (1984) 1146.
S. Gorman, C. Adair, F. O. O'Neill, C. Goldsmith and H. Webb, Eur. J. Clin. Microbiol. Infect. Diseases 12 (1) (1993) 9.
W. C. Hoffman, Contacto 27 (4) (1983) 3.
S. E. Tebbs, A. Sawyer and T. S. J. Elliott, Br. J. Anaesthesia 72 (1994) 587.
E. Budtz-Jorgensen, E. Theilade, J. Theilade and H. Zander, Scand. J. Dent. Res. 89 (1981) 149.
J. Verran, G. C. Lees and A. P. Shakespeare, Biofouling 3 (1991) 183.
E. A. Zottola and K. C. Sasahara, Int. Food Microbiol. 23 (1991) 125.
A. J. D. Romney, Soc. Dairy Technol. CIP (1980) 47.
J. Verran, D. B. Drucker and C. J. Taylor, Microbios 29 (1980) 161.
R. Locci, G. Peters and G. Pulverer, Zentralbratt Bakt. Mikrobiol. Hygiene (I. Abteilung) 173 (1981) 300.
T. Lie, Acta Odontol. Scand. 37 (1979) 73.
M. Quirynen, M. Marechal, D. van Steenberghe, H. J. Busscher and H. C. van der Mei, Biofouling 4 (1991) 187.
M, Quirynen, M. Marechal, H. J. Busscher, A. H. Weerkamp, J. Arends, P. L. Darius and D. van Steenberghe, J. Clin. Periodont. 17 (1990) 138.
J. Verran, R. L. Taylor and G. C. Lees, J. Mater. Sci. Mater. Med. 7 (1996) 597.
M. Bradford, Anal. Biochem. 72 (1976) 248.
J. Verran, R. L. Taylor and G. C. Lees, Binary 6 (1994) 55.
M. Yamauchi, K. Yamamoto, M. Wakabayashi and J. Kawano, Dent. Mater. J. 9 (1) (1990) 19.
H. J. Busscher, J. Sjollema and H. C. van der Mei, in “Microbial Cell Surface Hydrophobicity”, edited by R. J. Doyle and M. Rosenberg (American Society for Microbiology, Washington, DC, 1990) p. 335.
M. Quirynen and C. M. L. Bollen, J. Clin. Periodont. 22 (1995) 1.
R. N. Wenzel, Ind. Eng. Chem. 28 (1936) 988.
H. J. Busscher, A. W. J. van Pelt, P. de Boer, H. P. de Jong and J. Arends, Coll. Surfaces 9 (1984) 319.
H. N. Newman and D. F. G. Poole, Helv. Odontol. Acta 7 (1973) 58.
H. M. W. Uyen, J. M. Schakenaad, J. Sjollema, J. Noordmans, W. L. Jongebloed, I. Stokroos and H. J. Busscher, J. Biomed. Mater. Res. 24 (1990) 1599. ??
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TAYLOR, R.L., VERRAN, J., LEES, G.C. et al. The influence of substratum topography on bacterial adhesion to polymethyl methacrylate. Journal of Materials Science: Materials in Medicine 9, 17–22 (1998). https://doi.org/10.1023/A:1008874326324
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DOI: https://doi.org/10.1023/A:1008874326324