Abstract
The BIAcore biosensor was used to detect binding of Escherichia coli O157:H7 with specific antibodies. Immobilized Protein A or Protein G captured antibodies which in turn bound to the bacteria. Alternatively, immobilized antibody captured the E. coli O157:H7 and the bacteria were further probed by a second antibody which enhanced the signal. The regenerated sensor surfaces were used for at least 50 separate analyses. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with different antisera or other molecular species. © Rapid Science Ltd. 1998
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Fratamico, P., Strobaugh, T., Medina, M. et al. Detection of Escherichia coli 0157:H7 using a surface plasmon resonance biosensor. Biotechnology Techniques 12, 571–576 (1998). https://doi.org/10.1023/A:1008872002336
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DOI: https://doi.org/10.1023/A:1008872002336