Abstract
A non-destructive micro-test which allow the identification of homozygous and heterozygous soybean seeds for lipoxygenases (LOX) 1 and 3 has been developed based on determination of their enzymatic activities. Identification of heterozygous seeds will be extremely important in backcross breeding programme to expedite the creation of new soybean lines lacking seed lipoxygenases because no selfings are necessary during the odd numbered generations.
Similar content being viewed by others
References
Barros, E.G., Moreira, M.A., Fontes, E.P.B. (1984). Arq. Biol. Tecnol. 27, 381-386.
Kitamura, K., Davies, C. S., Kaizuma, N. and Nielsen, N.C. (1983). Crop Sci. 23, 924-927.
Kitamura, K. (1984). Agric. Biol. Chem. 48, 2339-2346.
Moreira, M.A., Rezende, S.T., Ramos, V. and Barros, E.G. (1993). J. Agric. Food Chem. 41, 103-106.
Rackis, J.J., Sessa, D.J. and Honig, D.H. (1979). J. Amer. Oil Chem. Soc. 56, 262-271.
Suda, I., Hajika, M., Nishida, Y., Furuta, S. and Igita, K. (1995). J. Agric. Food Chem. 43, 742-747.
Rights and permissions
About this article
Cite this article
Alves de Oliveira, D., Piovesan, N.D., Alves de Moraes, R.M. et al. Identification of the three genotypic classes for soybean lipoxygenases 1 and 3 based on enzymatic activity. Biotechnology Techniques 12, 71–74 (1998). https://doi.org/10.1023/A:1008863728043
Issue Date:
DOI: https://doi.org/10.1023/A:1008863728043