Skip to main content
Log in

Xylitol purification by cross-linked glucose isomerase crystals

  • Published:
Biotechnology Techniques

Abstract

Xylitol is specifically bound by active, cross-linked glucose isomerase crystals (CLGI). CLGI can be used to purify xylitol or concentrate it from dilute and impure solutions. Bound xylitol can be eluted from CLGI by Ca2 and the material reactivated by Mg2. The binding capacity is 1 mg xylitol per 525 mg CLGI which equals one molecule per active center. CLGI can further be used to purify xylitol and sorbitol from impure mixtures of arabinitol, mannitol, ribitol and monosaccharides. © Rapid Science Ltd. 1998

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Allenmark S, Bomgren B and Boren H (1983). J Chromatogr264: 63-68

    Google Scholar 

  • Bradford, MM (1979). Anal Biochem72: 248-254

    Google Scholar 

  • Converti, A and Del Borghi M (1998). Bioproc Eng18: 27-33

    Google Scholar 

  • De Lorenzi E, Massolini G, Lloyd DK, Monaco HL, Galbusera C and Caccialanza G (1997). J Chromatogr790: 47-64

    Google Scholar 

  • Hermansson, J (1983). J Chromatogr269: 71-80

    Google Scholar 

  • Margolin, AL (1996). TIBTECH14: 219-259

    Google Scholar 

  • Man, JC de, Rogosa, M. and Sharpe, ME (1960). J Appl Bact23: 130

    Google Scholar 

  • Narayanan SR (1992). J Pharm Biomed Anal10: 251-262

    Google Scholar 

  • Visuri, K (1989). United States Patent Application93-149158 931108 (8)

  • Visuri, K (1987). United States Patent4,699,882

  • Watt, CIF (1998). Hydride and alkyl group shifts in the reactions of aldehydes and ketones, in Comprehensive Biological Catalysis(Sinnott, M., eds), pp. 252-272, Academic Press Limited, Harcourt Brace and Company, New York.

    Google Scholar 

Download references

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pastinen, O., Visuri, K. & Leisola, M. Xylitol purification by cross-linked glucose isomerase crystals. Biotechnology Techniques 12, 557–560 (1998). https://doi.org/10.1023/A:1008863700518

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008863700518

Keywords

Navigation