Abstract
Bacillus megaterium is a potential bioremediation and biocontrol agent. The accumulation of reserve polymers, such as poly-3-hydroxybutyrate (PHB), increases survival of B. megaterium in water. We used wild-type strains of this species and mutant strains deficient in PHB synthesis in soil microcosms for testing the hypothesis that differences in survival capabilities and spore quality between strains is maintained in heterogeneous environments enriched with organic matter. No differences in survival between strains, nor a decrease in bacterial cell numbers were observed in sterile soil microcosms. In non-sterile soil, the total cell number (vegetative cells plus spores) of the PHB wild-type strain was 3.5 times higher than that of the PHB-negative mutant. We suggest that for predictive purposes, validation of survival in a variety of conditions is necessary.
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Bertagnolli, B.L., Dalsoglio, F.K. & Sinclair, J.B. 1996 Extracellular enzyme profiles of the fungal pathogen Rhizoctonia solani isolate 2B-12 and of two antagonists Bacillus megaterium strain b153-2-2 and Trichoderma harzianum isolate Th008. 1. Possible correlations with inhibition of growth and biocontrol. Physiological and Molecular Plant Pathology 48(3), 145–160.
Bohall Jr., N.A. & Vary, P.S. 1986 Transposition of Tn917 in Bacillus megaterium. Journal of Bacteriology 167, 716–718.
Clewell, D.B. & Gawron-Burke, C. 1988 Conjugative transposons and the dissemination of antibiotic resistance in streptococci. Annual Review of Microbiology 40, 635–665.
Floccari, M., Tiscornia, G., Palmada, F. & Méndez, B. 1991 Enriquecimiento en mutantes de Bacillus megaterium deficientes en la síntesis de poli(3-hidroxibutirato). Revista Argentina de Microbiología 23, 14–25.
Lafuente, R., Maym-Gatel, X., Mas-Castellá J. & Guerrero R. 1996 Influence of Environmental Factors on Plasmid Transfer in Soil Microcosms. Current Microbiology 32, 213–220.
López, N.I., Floccari, M.E., Steinbüchel, A., Garcia, A.F. & Méndez, B.S. 1995a Effect of poly(3-hydroxybutyrate) (PHB) content on the starvation-survival of bacteria in natural waters. FEMS Microbiology Ecology 16, 95–102.
López, N.I., Duarte, C.M., Vallespinós, F., Romero, J. & Alcoverro, T. 1995b Bacterial activities in seagrass (Posidonia oceanica) sediments. Journal of Experimental Marine Biology and Ecology 187, 39–49.
Ostle, A. & Holt, J.G. 1982 Nile Blue A as a fluorescent stain for poly—hydroxybutyrate. Applied and Environmental Microbiology 44(1), 238–241.
Postma, J., van Veen, J.A. & Walter S. 1989 Influence of different initial soil moisture contents on the distribution and population dynamics on introduced Rhizobium leguminosarum biovar trifolii. Soil Biology and Biochemistry 21, 437–442.
Postma, J., Hok-A-Hin, C.H., Schotman, J.M.T., Wijffelman, C.A. & van Veen, J.A. 1991 Population dynamics of Rhizobium leguminosarum Tn5 mutants with altered cell surface properties introduced into sterile and nonsterile soils. Applied and Environmental Microbiology 57, 649–654.
Quinn, J.P., Pedens, J.M.M. & Dick, R.E. 1989 Carbon-phosphorus bond cleavage by Gram-positive and Gram-negative soil bacteria. Applied Microbiology and Biotechnology 31, 283–287.
Saxena, A., Zhang, R.W. & Bollag, J.M. 1987 Microorganisms capable of metabolizing the herbicide metolachlor. Applied and Environmental Microbiology 53, 390–396.
Tao, Y-P. & Vary, P.S. 1991 Isolation and characterization of a sporulation lacZ fusion mutants of Bacillus megaterium. Journal of General Microbiology 137, 797–806.
van Elsas, J.D. & van Overbeek, L.S. 1992 Bacterial responses to soil stimuli. In Starvation in Bacteria, ed Kjelleberg, S. pp. 55–79. New York: Plenum Press.
Vary, P. 1994 Prime time for Bacillus megaterium. Microbiology 140, 1001–1013.
Wagner-Döbler, I., Pipke, R., Timmis, K.N. & Dwyer, D.F. 1992 Evaluation of aquatic sediment microcosms and their use in assessing possible effects of introduced microorganisms on ecosystem parameters. Applied and Environmental Microbiology 58(4), 1249–1258.
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López, N., Ruiz, J. & Méndez, B. Survival of poly-3-hydroxybutyrate-producing bacteria in soil microcosms. World Journal of Microbiology and Biotechnology 14, 681–684 (1998). https://doi.org/10.1023/A:1008857420434
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DOI: https://doi.org/10.1023/A:1008857420434