Abstract
A programme was created to semi-automatically analyse protozoal digitised images. Principal Component Analysis technique was used for species identification. After data collection and mathematical treatment, a three-dimensional representation was generated and several protozoa (Opercularia, Colpidium, Tetrahymena, Prorodon, Glaucoma and Trachelophyllum) species could be positively identified.
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Chandwani RM, Pate ME, Thornhill NF, Horstmann B, Hoare M, Titchener-Hooker NJ (1997) Bioprocess Eng. 17: 229-234.
Chen C, McAvoy TJ (1996) Can. J. Chem. Eng. 74: 1010-1024.
Curds CR, Cockburn A (1970) Water Res. 4: 237-249.
Einax JW, Zwanziger HW, Geiss S (1997) Chemometrics in Environmental Analysis. Weinheim: VCH.
Fenchel T (1987) Ecology of Protozoa. Berlin: Springer-Verlag.
Fernandez-Leborans G, Novillo A (1996) Water Environ. Res. 68: 311-319.
Jahn TL, Bovee EC, Jahn FF (1979) How to Know the Protozoa. Dubuque, IO: Wm. C. Brown Company Publishers.
Lynn DH (1992) J. Aquatic Ecosystem Health 1: 263-270.
Macek M (1989) Int Revue Ges Hidro-Biology 74: 643-656.
Madoni P (1994) Water Res. 28: 67-75.
Madoni P, Davdi D, Chieriici E (1993) Water Res. 27: 1485-1491.
Pichon D, Vivier H, Pons MN (1992) In: Karim NM, Stephanopoulos G, eds. Modeling and Control of Biotechnical Process. London: Pergamon Press, pp. 307-309.
Pons MN, Mona H, Drouin JF, Vivier H (1995) In: Munack A, Schügerl K, eds. Postprints of the 6th Int. Conf. on Computer Applications in Biotechnology. London: Pergamon Press, pp. 189-194.
Pons MN, Wagner A, Vivier H, Marc A (1992) Biotechnol. Bioeng. 40: 187-193.
Pons MN, Vivier H, Rémy JF, Dods JA (1993) Biotechnol. Bioeng. 42: 1352-1359.
Russ JC (1995). The Image Processing Handbook. Boca Raton, FL: CRC Press.
Salvadó H, Gracia MP, Amigó JM (1995) Water Res. 29: 1041-1050.
Sudo R, Aiba S (1984) Adv. Biochem. Eng. 29: 117-141.
Vecht-Lifshitz SE, Ison AP (1992) J. Biotechnol. 23: 1-18.
Yun-Fen S, Buikema AL, Vongue Jr WH, Pratt JR, Cairns Jr J (1986) J. Protozool. 33: 146-151.
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Amaral, A., Baptiste, C., Pons, M. et al. Semi-automated recognition of protozoa by image analysis. Biotechnology Techniques 13, 111–118 (1999). https://doi.org/10.1023/A:1008850701796
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DOI: https://doi.org/10.1023/A:1008850701796