Abstract
Spherical gel beads of collagen/alginate were prepared by discharging droplets of a mixture containing collagen (1.07-1.9 mg/ml) and alginate (1.2-1.5% w/v) into 1.5% w/v CaCl2 solution at 4°C. Collagen in the gel beads was reconstituted by raising the temperature to 37°C after alginate was liquefied by citrate. Scanning electron microscopy of the beads revealed the characteristic fibrous structure of collagen. To demonstrate the application of this new technique in cell culture, GH3 rat pituitary tumor cells were entrapped and grown in the gel beads. The immobilized cells proliferated to a density of 1.95 x 106 cell/ml which is about an order of magnitude higher than that grown in the alginate beads.
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References
Altankov, G., Brodvarova, I., and Rashkov, I. (1991). J. Biomat. Sci.-Polym. E., 2: 81-89
Cahn, F. (1990). Trends Biotechnol., 8: 131-136
Dean, R. C. (1989). US Patent, 4,863,856
Huang-Lee, L.L.H., and Nimni, M.E. (1993). Biomed. Eng.-Applications, Basis & Communications, 5(5): 60-71
Langer, R. and Vacanti, J. P. (1993). Science, 260: 920-926
Smidsrod, O. and Skjak-Brek, G. (1990). Trends Biotechnol., 8: 71-78
Thu, B., Bruheim, P., Espevik, T., Smidsrod, O., Soon-Shiong, P. and Skjak-Brak, G. (1996). Biomaterials, 17: 1031-1040
Yannas, I.V. and Kirk, J.F. (1984). US Patent, 4,448,718
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Tsai, S.W., Jeng, M.J., Tsay, R.Y. et al. Gel beads composed of collagen reconstituted in alginate. Biotechnology Techniques 12, 21–23 (1998). https://doi.org/10.1023/A:1008843223500
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DOI: https://doi.org/10.1023/A:1008843223500