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Preparation of pure monoclonal antibody to interleukin-2 by cultivation of hybridoma cells entrapped in novel composite hydrogel beads

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Biotechnology Techniques

Abstract

A simple and reliable technique was developed to prepare pure monoclonal antibody (MAb) to interleukin-2 using cells entrapped in novel composite poly(N-vinyl caprolactam)-calcium alginate beads. Flow cytometry was applied to study cell size and cell cytoplasm granularity distribution. Maximum MAb production by the gel-entrapped cells in serum free medium was 2-3-fold higher compared to free suspension culture in serum containing medium. The only contaminating protein in culture supernatant was transferrin at 5% w/v.

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Authors
  1. A.A. Vikhrov
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  2. E.A. Markvicheva
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  3. T.Yu. Mareeva
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  4. S.V. Khaidukov
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  5. V.A. Nesmeyanov
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  6. M.N. Manakov
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  7. J-L. Goergen
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  8. A. Marc
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  9. V.P. Zubov
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Vikhrov, A., Markvicheva, E., Mareeva, T. et al. Preparation of pure monoclonal antibody to interleukin-2 by cultivation of hybridoma cells entrapped in novel composite hydrogel beads. Biotechnology Techniques 12, 11–14 (1998). https://doi.org/10.1023/A:1008839122591

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  • DOI: https://doi.org/10.1023/A:1008839122591

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