Abstract
Two truncated Bacillus thuringiensis δ-endotoxin genes, belonging to the classes cry1Ab and cry1B, and both coding for N-terminal toxic fragments of the corresponding crystal proteins, were translationally fused. Expression of the fusion gene driven by the cry1C promoter in Escherichia coli at a very high level resulted in a protein with enhanced toxicity to the diamondback moth (Plutella xylostella).
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References
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Mandaokar, A., Chakrabarti, S., Rao, N. et al. A fusion gene coding for two different δ-endotoxinsof Bacillus thuringiensis toxic to Plutella xylostella and usefulfor resistance management. World Journal of Microbiology and Biotechnology 14, 599–601 (1998). https://doi.org/10.1023/A:1008821023334
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DOI: https://doi.org/10.1023/A:1008821023334