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Determination of aflatoxin levels in pistachio and cashew nuts using immunoaffinity column clean-up with HPLC and fluorescence detection

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Biotechnology Techniques

Abstract

A technique for the detection of aflatoxins in pistachio and cashew nuts using immunoaffinity column clean-up with HPLC and fluorescent detection is presented. Recoveries were in the range of 79–99% for pistachio samples artificially contaminated with 10 μg total aflatoxins kg−1 of food sample. For cashew samples recoveries ranged from 80–106%. This method is proposed as an accurate technique for aflatoxin detection in the range of μg aflatoxins kg−1 nuts.

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Authors and Affiliations

  1. School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow, G4 OBA, Scotland

    S.M. Pearson, A.A.G. Candlish & K.E. Aidoo

  2. Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, G1 1XW, Scotland

    J.E. Smith

Authors
  1. S.M. Pearson
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  2. A.A.G. Candlish
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  3. K.E. Aidoo
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  4. J.E. Smith
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Pearson, S., Candlish, A., Aidoo, K. et al. Determination of aflatoxin levels in pistachio and cashew nuts using immunoaffinity column clean-up with HPLC and fluorescence detection. Biotechnology Techniques 13, 97–99 (1999). https://doi.org/10.1023/A:1008819914690

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  • DOI: https://doi.org/10.1023/A:1008819914690

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