Skip to main content
Log in

Biodegradation of olive oil mill wastewaterby Coriolus versicolor and Funalia trogii:effects of agitation, initial COD concentration, inoculum size and immobilization

  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The biodegradation of olive oil mill wastewater (OOMW) by Coriolus versicolor and Funalia trogii was investigated. Initial COD concentration, agitation and inoculum size were all found to be significant for biodegradation. Adding glucose, sulphate or nitrogen had no effect on biodegradation. During growth in optimum conditions, C.versicolor removed approximately 63% COD, 90% phenol and 65% colour within 6 days and F. trogii removed approximately 70% COD, 93% phenol and 81% colour of the OOMW used. The fungi also excreted large amounts of extracellular laccase into the medium. High biodegradation yields were also obtained by fungi immobilized in calcium alginate gels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Archibald, F., Paice, M.G. and Jurasek, L. 1990 Decolorization of Kraft bleachery effluent chromophores by Coriolus (Trametes) versicolor. Enzyme and Microbial Technology. 12, 846–853.

    Google Scholar 

  • Arora, S.D. & Sandhu, K.D. 1985 Laccase production and wood degradation by a white rot fungus Daedalea flavida. Enzyme and Microbial Technology 7, 405–408.

    Google Scholar 

  • Chivukula, M. & Renganathan, V. 1995 Phenolic azo dye oxidation by laccase from Pyricularia oryzae. Applied and Environmental Microbiology. 61, 4374–4377.

    Google Scholar 

  • Eikmeier, H., Westmeier, F. & Rhm, H.J. 1984 Morphological development of Aspergillus niger immobilized in Ca-alginate and K-carrageenan. AppliedMicrobiology and Biotechnology. 19, 53–57

    Google Scholar 

  • Gonzales, D.M., Moreno, E., Sarmiento, Q.J. & Cormenzana, R.A. 1990 Studies on antibacterial activity of wastewaters from olive oil mills (Alpechin): Inhibitory activity of phenolic and fatty acids. Chemosphere 20, 423–432.

    Google Scholar 

  • Hamdi, M. 1993 Future prospect and constraints of olive mill wastewater use and treatment: A Review. Bioprocess Engineering 8, 209–214.

    Google Scholar 

  • Hamdi, M. & Garcia, L.J. 1993 Anaerobic digestion of olive mill wastewaters after detoxification by prior culture of Aspergillus niger. Process Biochemistry 28, 155–159.

    Google Scholar 

  • Hamdi, M., Kahdir, A. & Garcia, L.J. 1991 The use of Aspergillus niger for the bioconversion of olive mill waste-waters. Applied Microbiology and Biotechnology. 34, 828–831.

    Google Scholar 

  • Hammel, K.E. 1989 Organopollutant degradation by lignolytic fungi. Enzyme and Microbial Technology. 11, 776–777.

    Google Scholar 

  • Martirani, L., Giardina, P., Marzullo, L. & Sannia, G. 1996 Reduction of phenol content and toxicity in olive oil mill waste waters with the ligninolytic fungus Pleurotus ostreatus. Water Research 30(8), 1914–1918.

    Google Scholar 

  • Miller, G.L. 1959 Use of dinitrosalicilic acid reagent for determination of reducing sugar. Analytical Chemistry 31, 426–428

    Google Scholar 

  • Milstein, O., Hüttermann, A., Majcherczyk, A. & Schulze K. 1993 Transformation of lignin-related compounds with laccase in organic solvents. Journal of Biotechnology 30, 37–47.

    Google Scholar 

  • Paice, G.M., Bourbonnas, R., Reid, D.I., Archibald, S.F. & Jurasek, L. 1995 Oxidative bleaching enzymes: a review. Journal of Pulp and Paper Science 21, 280–284.

    Google Scholar 

  • Perez, D.J., Esteban, E., Gomez, M. & Gallardo-Lara, F. 1986 Effects of wastewater from olive processing on seed germination and early plant growth of different vegetable species. Environmental Science and Health, B21(4), 349–357.

    Google Scholar 

  • Reid, D.I. & Paice, G.M. 1994 Biological bleaching of kraft pulps by white-rot fungi and their enzymes. FEMS Microbiological Reviews 13, 369–376.

    Google Scholar 

  • Rodriguez, M.M.A., Perez, J., Ramos-Cormenzana, A. & Martinez, J. 1988 Effect of extracts obtained from olive oil mill waste waters on Bacillus megaterium ATCC 33085. Journal of Applied Bacteriology 64, 219–226.

    Google Scholar 

  • Rosenberg, S.L. 1980 Physiological studies of lignocellulose degradation by the Thermotolerant Mold Chrysosporium prunosum. Symposium on the Biological Transformation of Lignocellulose 12, 133–142.

    Google Scholar 

  • Roy-Arcand, L. & Archibald, S.F. 1991 Direct dechlorination of chlorophenolic compounds by laccase from Trametes (Coriolus) versicolor. Enzyme and Microbial Technology 13, 194–203.

    Google Scholar 

  • Sanjust, E., Pompei, R., Resciggno, A., Augusto, R. & Ballero, M. 1991 Olive milling wastewater as a medium for growth of four Pleurotus species. Applied Biochemistry and Biotechnology 31, 223–235.

    Google Scholar 

  • Standard Methods. 1979 Standard methods for the examination of water and wastewater. Fourteenth Edition American Public Health Association.

  • Vinciguerra, V., D'annibale, A., Monache, G.D. & Sermanni, G.G. 1995 Correlated effects during the bioconversion of waste olive waters by Lentinus edodes. Bioresource Technology 51, 221–226.

    Google Scholar 

  • Yesilada, Ö., 1995 Decolorization of crystal violet by fungi. World Journal of Microbiology and Biotechnology 11, 601–602.

    Google Scholar 

  • Yesilada, Ö. & Fiskin, K. 1995 The use of white rot fungi for evaluation and degradation of vinasse. Turkish Journal of Biology 19, 181–189.

    Google Scholar 

  • Yesilada, Ö., & Fiskin, K. 1996 Degradation of olive mill waste by Coriolus versicolor. Turkish Journal of Biology 20, 73–79.

    Google Scholar 

  • Yesilada, Ö., Fiskin, K. & Yesilada, E. 1995 The use of white rot fungus Funalia trogii (Malatya) for the decolourization and phenol removal from olive oil mill wastewater. Environmental Technology 16, 95–100.

    Google Scholar 

  • Yoropolov, I.A., Skorobogat'ko, V.O., Vartanov, S. & Vorfolomeyev, D.S. 1994 Laccase: properties, catalytic mechanism, and applicability. Applied Biochemistry and Biotechnology 49, 257–280.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yesilada, O., Sik, S. & Sam, M. Biodegradation of olive oil mill wastewaterby Coriolus versicolor and Funalia trogii:effects of agitation, initial COD concentration, inoculum size and immobilization. World Journal of Microbiology and Biotechnology 14, 37–42 (1997). https://doi.org/10.1023/A:1008816231563

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008816231563

Navigation