Skip to main content
SpringerLink
Log in

Optimization of critical parameters for immobilization of Streptomyces clavuligerus on alginate gel matrix for cephamycin C production

  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The optimum critical parameters for immobilization of Streptomyces clavuligerus on alginate gel matrix for cephamycin C production, i.e. sodium alginate (wt. %), CaCl2 (M) and cell concentration (wt. %), curing time (h.), for enhanced gel stability, were obtained employing a full factorial search. The results indicate that the concentrations of CaCl2 and inoculum size were found to have a pronounced effect on cephamycin C fermentation. On the other hand, the higher concentration of sodium alginate exerted an adverse influence either individually or in combination with other variables. The path steepest ascent method has been used to optimize the variables. The optimum concentrations of matrix components were 3.218% sodium alginate, 0.996 M CaCl2, 19.06% cell concentration and 17.16 h. of curing time supported higher cephamycin C production, at 48 h. of fermentation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arcuri, E.J., Sla., G. & Greasham, R. 1986 Continuous production of theinamycin in immobilized cell systems. Biotechnology and Bioengineering 28, 842–849.

    Google Scholar 

  • Akhnazarova, S., & Kafarov, V. 1982 Experiment Optimization in Chemistry and Chemical Engineering. Mir publishers, Moscow. pp. 151–171.

    Google Scholar 

  • Kavanagh. F. (edr.) 1963 In: Analytical Microbiology - 1 Kavanagh Academic Press. pp. 313–346.

  • Keshvarz, T., Eglin, R., Walker, E., Bucke, C., Holt, G., Bull, A.T. & Lilly, M.D. 1990 The large scale immobilization of Pencillium chryosogenum: Batch and continuous operation in an air-lift reactor. Biotechnology and Bioengineering 36, 763–770.

    Google Scholar 

  • Freeman, A. & Aharonowitz, Y. 1981 Immobilization of microbial cells in cross linked, prepolymerized, linear polyacrylamide gels: antibiotic production by immobilized Streptomyces clavuligerus cells. Biotechnology and Bioengineering 23, 2747–2759.

    Google Scholar 

  • Martinsen, A., Skjak-Brack, G. & Shidsrol, O. 1989 Alginate as immobilized material, Correlation between chemical and physical properties of alginate gel beads. Biotechnology and Bioengineering 33, 79–89.

    Google Scholar 

  • Morikawa, Y., Karube, I. & Suzuki. S. 1979 Penicillin G production by immobilized whole cells Penicillium chrysogenum. Biotechnology and Bioengineering 21, 261–270.

    Google Scholar 

  • Mina Dalili, 1988 Production of actinomycin D with immobilized Streptomyces parvullus under nitrogen and carbon starvation conditions. Biotechnonogy Letters 10, 40–45.

    Google Scholar 

  • Nagarjan, R., Boeck, L.D., Gorman, M., Hamill, R.L., Higgens, C.E., Hoehn, M.M., Stark, W.M. & Whitney, J.G. 1971 β-Lactam antibiotics from Streptomyces. Journal of American Chemical Society 93, 2308–2312.

    Google Scholar 

  • Obgonna, J.C., Amano, Y. & Nakamura, K. 1989 Elucidation of optimum conditions for immobilization of viable cells by using calcium alginate. Journal of Fermentation and Bioengineering 67, 92–96.

    Google Scholar 

  • Omstead, D.R., Hunt, G.R. & Buckland, B.C. 1986 Commercial production of cephamycin antibiotics. In: Comprehensive Biotechnology (ISBN 0–08–032511–4) M. Moo Young (edr.) Pergamon Press 3, pp. 187–270.

  • Ramakrishna, S.V., Sreedharan, V. P. & Prema, P. 1988 Continuous ethanol production with immobilized yeast cells in a packed bed reactor, In: Moo Young, M. (ed.), Bioreactor Immobilized Enzymes and Cells: Fundamentals and applications-I. Elsevier Applied Science publishers, Amsterdam. pp. 251–260. ISBN 1–85–166160–3.

    Google Scholar 

  • Ramakrishna, S.V., Ratnakumari, T.S. & Sai, P.S.T. 1991 Optimization of process parameters for the continuous production of alcohol using cell immobilized bioreactor. Bioprocess Engineering 6, (4) 141–144.

    Google Scholar 

  • Stapley, E.O. & Birnbaum. J. 1981 Chemistry and microbiological properties of the cephamycins. In: β-Lactam Antibiotics ed. Salton, M.R.J. & Shockman, G.D. Academic press. New York. pp. 327–351. ISBN 0–12–616050–3.

    Google Scholar 

  • Sri devi, V. 1998 Cephamycin C production by alginate immobilized cells. In: Production of cephamycin C from immobilized Streptomyces clavuligerus (Ph.D thesis) Osmania University, pp. 100–200.

  • Veelken, M. & Pape, H. 1982 Production of tylosin and nikkomycin by immobilized Streptomyces cells. Europian Journal of Applied Microbiology and Biotechnology 15, 206–210.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Osmania University, Hyderabad -, 500 007, A.P., India

    Sri Devi & Padma Sridhar

Authors
  1. Sri Devi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Padma Sridhar
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Devi, S., Sridhar, P. Optimization of critical parameters for immobilization of Streptomyces clavuligerus on alginate gel matrix for cephamycin C production. World Journal of Microbiology and Biotechnology 15, 185–192 (1999). https://doi.org/10.1023/A:1008814427427

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008814427427

Search

Navigation