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Growth promotion by yeastolate and related components on insect cells

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Biotechnology Techniques

Abstract

In this study, we tested a number of defined and undefined compounds in comparison with yeastolate in Sf-9 insect cell culture including a group of B vitamins and a series of purines and pyrimidines. Yeastolate promoted the cell growth appreciably, increasing the maximum cell density by more than 60%. Vitamins and purines, which are considered as the major constituents of yeastolate, are not responsible for its growth-promoting effect.

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References

  • Bédard, C, Kamen, A, Tom, RL and Massie, B (1994) Cytotechnol. 15, 129-138

    Google Scholar 

  • Drews, M, Paalme, T and Vilu, R (1995) J. Biotechnol. 40, 187-198

    Google Scholar 

  • Kelley, WN (1972) Purine and pyrimidine metabolism of insect cells in culture. In: Rothblat, G.H. and Cristofalo, V.J. (eds), Growth, Nutrition and Metabolism of Cells in Culture, vol.1, pp211-255, Academic Press, New York

    Google Scholar 

  • Kurti, TJ and Munderloh, UG (1989) Advances in the definition of culture media for mosquito cells. In: Mitsuhashi, J (ed) Invertebrate Cell System Applications, vol.1, pp21-29, CRC Press, Inc., Boca Raton, Fla., USA

    Google Scholar 

  • Lee, SH and Park, TH (1994) Biotechnol. Lett. 16, 327-332

    Google Scholar 

  • Luckow, VA (1991) Cloning and expression of heterologous genes in insect cells with baculovirus vectors. In: Prokop, A, Bajpai, RK and Ho, CS (eds), Recombinant DNA Technology and Applications, pp97-152, McGraw-Hill, New York

    Google Scholar 

  • Mitsuhashi, J (1995) In Vitro Cell Dev Biol-Animal, 31, 822-823

    Google Scholar 

  • Mosna, G (1981) Experientia, 37, 466-467

    Google Scholar 

  • Nagasawa, H, Mitsuhashi, J and Suzuki, A (1987) Growth factors in the lactalbumin hydrolysate to the cells of the flesh fly, Sarcophaga peregrina. In: Kuroda, Y., Kurstak, E. and Maramorosch, K. (eds), Invertebrate and Fish Tissue Culture, pp29-32, Japan Sci. Soc. Press, Springer-Verlag, Tokyo

    Google Scholar 

  • Nguyen, B, Jarnagin, K, Williams, S, Chan, H and Barnett, J (1993) J. Biotechnol. 31, 205-217

    Google Scholar 

  • Reuveny, S, Kim, YJ, Kemp, CW and Shiloach, J (1993) Biotechnol. Bioeng. 42, 235-239

    Google Scholar 

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Wu, J., Lee, Kl.D. Growth promotion by yeastolate and related components on insect cells. Biotechnology Techniques 12, 67–70 (1998). https://doi.org/10.1023/A:1008811711205

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  • DOI: https://doi.org/10.1023/A:1008811711205

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