Abstract
An immortalized B-cell library consisting of cells secreting antibodies to food allergens has been prepared. Peripheral B-cells from seven healthy donors were transformed with Epstein-Barr virus and an immortalized human B-cell library with 2,202 ampoules of multi-clone B-lymphoblastoid cells was obtained. The B-cell library contained various types of B-lymphoblastoid cells secreting antibodies to rice, soybeans, milk, and eggs, and can be used to analyze food allergens. © Rapid Science Ltd. 1998
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Shinmoto, H., Nakahara, K., Kobori, M. et al. Preparation of human immortalized B-cells secreting antibodies against food allergens. Biotechnology Techniques 12, 545–547 (1998). https://doi.org/10.1023/A:1008807615539
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DOI: https://doi.org/10.1023/A:1008807615539