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Biotechnology Techniques

, Volume 12, Issue 10, pp 793–796 | Cite as

Optimization of transgene expression in sugar-cane cells

  • Joel González-Cabrera
  • Alberto Coego-González
  • Ariel F. Martínez-Gil
  • Gustavo A. de la Riva
  • Roberto I. Vázquez-Padrón
Article

Abstract

Sugar-cane cells were transformed by electroporation using different genetic constructs to analyze the influence of the octopine synthetase gene enhancer (ocs) and the first intron-exon from rice act1 gene (a–E/I/E), upon gusA transgene expression. The quadruplicated ocs enhancer (4ocs) increased 6-fold the expression of the reporter gene. The introduction of the a-E/I/E into the transcriptional unit of the 4ocs/35S::gusA chimeric gene increased β-glucuronidase (Gus) activity more than 69 times in transformed sugar-cane cells. The lack of the 5'end of the a-E/I/E sequence led to a drastic decrease in the expression levels of the reporter gene. © Rapid Science Ltd. 1998

Keywords

Reporter Gene Act1 Gene Drastic Decrease Chimeric Gene Transcriptional Unit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall 1998

Authors and Affiliations

  • Joel González-Cabrera
    • 1
  • Alberto Coego-González
    • 1
  • Ariel F. Martínez-Gil
    • 1
  • Gustavo A. de la Riva
    • 1
  • Roberto I. Vázquez-Padrón
    • 1
  1. 1.Department of Environment Biotechnology, Plant DivisionCenter for Genetic Engineering and Biotechnology (CIGB)HavanaCuba

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