Abstract
An immunogold labelling technique has been developed and used to characterize the localization of mosquito larvicidal binary toxin in Bacillus sphaericus 2362, asporogenous mutants of Bacillus sphaericus 2362, Bacillus megaterium pWH1520 TK and E. coli DH5α pAR5. Different patterns of binary toxin concentration occur between the wild type and recombinant strains which may be related to their effective toxicities. © Rapid Science Ltd. 1998
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References
Baumann, P., Clark, M.A., Baumann, L. and Broadwell, A.H. (1991). Microbiol Rev 55: 425-436.
Bourgouin, C., Delecluse, A., de la Torre, F. and Szulmajster, J. (1990). Appl Environ Microbiol 56: 340-344.
Broadwell, A.H., Baumann, L. and Baumann, P. (1990). J Bacteriol 172: 2217-2223.
Charles, J.-F., Kalfon, A., Bourgouin, C. and de Barjac, H. (1988). Ann Inst Pasteur Microbiol 139: 243-259.
Charles, J.-F., Hamon, S. and Baumann, P. (1993). Res Microbiol 144: 411-416.
Cole, L., Hawes, C. and Dewey, F.M. (1991). Mycology Res 95: 1369-1374.
Davidson, E.W., Oei, C., Meyer, M., Bieber, A.L., Hindley, J. and Berry, C. (1990). Can J Microbiol 36: 870-878.
England, D.F., Penfold, R.J., Delaney, S.F. and Rogers, P.L. (1997). Curr Microbiol 35: 71-76.
Jiaviriyaboonya, S., Penfold, R.J. and Rogers, P.L. (1997). Aust Microbiol 18: A121.
Jiaviriyaboonya, S., Penfold, R.J. and Rogers, P.L. (1998). Curr Microbiol 36: 1-7.
Liu, J.-W., Porter, A.G., Wee, B.Y. and Thanabalu, T. (1996). Appl Environ Microbiol 62: 2174-2176.
de Marsac, N.T., de la Torre, F. and Szulmajster, J. (1987). Mol Gen Genet 209: 396-398.
Mulligan, F.S., Schaefer, C.H and Wilder, W.H. (1980). J Econ Entomol 73: 684-688.
Myers, P., Yousten, A.A. and Davidson, E.W. (1979). Can J Microbiol 25: 1227-1231.
Nicolas, L., Dossou-Yovo, J. and Hoygard, J-M. (1987). Appl Microbiol Biotechnol 25: 341-345.
Oeda, K., Inouye, K., Ibuchi, Y., Oshie, K., Shimizu, M., Nakamura, K., Nishioka, R., Takada, Y. and Ohkawa, H. (1989). J Bacteriol 171: 3568-3571.
Osborne, K.J. (1995) Ph.D. thesis, Growth, Sporulation and Larvicidal Activity of the Mosquito Pathogen Bacillus sphaericus2362 grown in Complex and Defined Media, UNSW, Sydney, Australia.
Priest, F.G. (1992). J Appl Bacteriol 72: 357-369.
Sangthongpitag, K., Penfold, R.J., Delaney, S.F. and Rogers, P.L. (1997). Appl Microbiol Biotechnol 47: 379-384.
Thanabalu, T., Hindley, J., Brenner, S., Oei, C. and Berry, C. (1992). Appl Environ Microbiol 58: 905-910.
Thanabalu, T., Hindley, J., Jackson-Yap, J. and Berry, C. (1991). J Bacteriol 173: 2776-2785.
Thanabalu, T. and Porter, A.G. (1996). Gene 170: 85-89.
Tommassen, J., Leunissen, J., Jongston, M.D. and Overduin, P. (1985). EMBO J 4: 1041-1047.
Xudong, X., Renqiu, K. and Yuxiang, H. (1993). FEMS Microbiol Lett 107: 247-250.
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Jiaviriyaboonya, S., Marks, P., England, D. et al. Mosquito larvicidal binary toxin localization in recombinant bacteria: identification using an immunogold labelling technique. Biotechnology Techniques 12, 533–538 (1998). https://doi.org/10.1023/A:1008803514631
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DOI: https://doi.org/10.1023/A:1008803514631