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Different Patterns of Host Genes are Induced in Rice by Pseudomonas syringae, a Biological Inducer of Resistance, and the Chemical Inducer Benzothiadiazole (BTH)

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Abstract

Rice seedlings treated with the synthetic compound benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH) acquired resistance to subsequent attack by the rice blast fungus Magnaporthe grisea (Hebert) Barr. BTH (trade name Bion™) has been released to the market as a plant protecting agent for rice. Here, we analysed the pattern of expressed genes in rice plants treated with BTH, and compared this pattern with those induced by the formerly discovered resistance inducer 2,6-dichloroisonicotinic acid (INA) and by Pseudomonas syringae pv. syringae, a non-host pathogen inducing a hypersensitive response. Both INA and BTH induced similar patterns of genes, suggesting that these compounds are functional analogues. In contrast, the patterns induced by the chemical inducers and by P. syringae were clearly dissimilar.

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References

  • Benhamou N and Bélanger RR (1998) Induction of systemic resistance to Pythium damping-off in cucumber plants by benzothiadiazole — Ultrastructure and cytochemistry of the host response. Plant J 14: 13-21

    Google Scholar 

  • Cao H, Bowling SA, Gordon S and Dong X (1994) Characterisation of an Arabidopsis mutant that is non-responsive to inducers of systemic acquired resistance. Plant Cell 6: 1583-1592

    Google Scholar 

  • Dann E, Diers B, Byrum J and Hammerschmidt R (1998) Effect of treating soybean with 2,6-dichloroisonicotinic acid (INA) and benzothiadiazole (BTH) on seed yields and the level of disease caused by Sclerotinia sclerotiorum in field and green-house studies. Eur J Plant Pathol 104: 271-278

    Google Scholar 

  • Delaney TP, Friedrich L and Ryals JA (1995) Arabidopsis signal transduction mutant defective in chemically and biologically induced disease resistance. Proc Natl Acad Sci USA 92: 6602-6606

    Google Scholar 

  • Epple P, Apel K and Bohlmann H (1995) An Arabidopsis thaliana thionin gene is inducible via a signal transduction pathway different from that for pathogenesis-related proteins. Plant Physiol 109: 813-820

    Google Scholar 

  • Frendo P, Didierjean L, Passelegue E and Burkhard G (1992) Abiotic stresses induce a thaumatin-like protein in maize. Plant Science 85: 61-69

    Google Scholar 

  • Friedirch L, Lawton K, Ruess W, Masner P, Specker N, Gut Rella M, Meier B, Dincher S, Staub T, Uknes S, Métraux J-P, Kessmann H and Ryals J (1996) A benzothiaidiazole derivate induces systemic acquired resistance in tobacco. Plant J 10: 61-70

    Google Scholar 

  • Görlach J, Volrath S, Knauf-Beiter G, Hengy G, Beckhove U, Kogel K-H, Oostendorp M, Staub T, Ward E, Kessmann H and Ryals J (1996) Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8: 629-643

    Google Scholar 

  • Jensen BD, Latundedada AO, Hudson D and Lucas JA (1998) Protection of Brassica seedlings against downy mildew and damping-off by seed treatment with CGA 245704, an activator of systemic acquired resistance. Pesticide Sc 52: 63-69

    Google Scholar 

  • Kastner B, Tenhaken R and Kauss H (1998) Chitinase in cucumber hypocotyls is induced by germinating fungal spores and by fungal elicitor in synergism with inducers of acquired resistance. Plant J 13: 447-454

    Google Scholar 

  • Katz VA, Thulke OU and Conrath U (1998) A benzothiadiazole primes parlsey cells for augmented elicitation of defence responses. Plant Physiol 117: 1333-1339

    Google Scholar 

  • Kuc J (1982) Induced immunity to plant disease. BioScience 32: 854-860

    Google Scholar 

  • Lawton K, Friedrich L, Hunt M, Weymann K, Delaney T, Kessmann H, Staub T and Ryals J (1996) Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway. Plant J 10: 71-82

    Google Scholar 

  • Métraux J-P, AhI-Goy P, Staub T, Speich J, Steinemann A, Ryals J and Ward E (1991) Induced systemic resistance in cucumber in response to 2,6-dichloro-isonicotinic acid and pathogens. In: Hennecke H and Verma DPS (eds) Advances in Molecular Genetics of Plant-Microbe Interactions. Vol 1 (pp. 432-439). Kluwer Academic Publishers, Dordrecht, Netherlands

    Google Scholar 

  • Midoh N and Iwata M (1996) Cloning and characterisation of a probenazole-inducible gene for an intracellular pathogenesis-related protein in rice. Plant Cell Physiol 37: 9-18

    Google Scholar 

  • Nielsen KK, Bojsen K, Collinge DB and Mikkelsen JD (1994) Induced resistance in sugar beet against Cercospora beticola: induction by dichloroisonicotinic acid is independent of chitinase and β-1,3-glucanase transcript accumulation. Physiol Mol Plant Pathol 45: 89-99

    Google Scholar 

  • Ouchi S, Oku H, Hibino C and Akiyama I (1974) Induction of accessibility and resistance in leaves of barley by some races of Erysiphe graminis. Phytopathol Zeitschr 79: 24-34

    Google Scholar 

  • Penninckx IAMA, Eggermont K, Terras FRG, Thomma BPHJ, De Samblanx GW, Buchala A, Metraux J-P, Manners JM and Broekaert WF (1996) Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway. Plant Cell 8: 2309-2323

    Google Scholar 

  • Pieterse CMJ, van Wees SCM, van Pelt JA, Knoester M, Laan R, Gerrits J, Weisbeek PJ and van Loon LC (1998) A novel signalling pathway controlling induced systemic resistance in Arabidopsis. Plant Cell 10: 1571-1580

    Google Scholar 

  • Reimmann C and Dudler R (1993) cDNA cloning and sequence analysis of a pathogen-induced thaumatin-like protein from rice (Oryza sativa). Plant Physiol 101;1113-1114

    Google Scholar 

  • Reimmann C, Hofmann C, Mauch F and Dudler R (1995) Characterization of a rice gene induced by Pseudomonas syringae pv. syringae: Requirement of the bacterial lemA gene function. Physiol Mol Plant Pathol 46: 71-81

    Google Scholar 

  • Reimmann C, Ringli C and Dudler R (1992) Complementary cDNA cloning and sequence analysis of a pathogen-induced putative peroxidase from rice. Plant Physiol 100: 1611-1612

    Google Scholar 

  • Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner H-Y and Hunt MD (1996) Systemic acquired resistance. Plant Cell 8: 1809-1819

    Google Scholar 

  • Schaffrath U, Freydl E and Dudler R (1997) Evidence for different signalling pathways activated by inducers of acquired resistance in wheat. Mol Plant-Microbe Interact 10: 779-783

    Google Scholar 

  • Schweizer P, Buchala A and Métraux J-P (1997a) Gene expression patterns and levels of jasmonic acid in rice treated with the resistance inducer 2,6-dichloro-isonicotinic acid. Plant Physiol 115: 61-70

    Google Scholar 

  • Schweizer P, Buchala A, Silverman P, Seskar M, Raskin I and Metraux J-P (1997b) Jasmonate-inducible genes are activated in rice by pathogen attack without a concomitant increase in endogenous jasmonic acid levels. Plant Physiol 114: 79-88

    Google Scholar 

  • Schweizer P, Hunziker W and Mösinger E (1989) cDNA cloning, in vitro transcription and partial sequence analysis of mRNAs from winter wheat (Triticum aestivum L.) with induced resistance to Erysiphe graminis f.sp. tritici. Plant Mol Biol 12: 643-654

    Google Scholar 

  • Schweizer P, Vallélian-Bindschedler, L and Mòsinger E (1995) Heat-induced resistance in barley to the powdery mildew fungus Erysiphe graminis f.sp. hordei. Physiol Mol Plant Pathol 47: 51-66

    Google Scholar 

  • Siegrist J, Glenewinkel D, Kolle C and Schmidtke M (1997) Chemically induced resistance in green bean against bacterial and fungal pathogens. J Plant Diseases Prot 104: 599-610

    Google Scholar 

  • Smith J and Métraux J-P (1991) Pseudomonas syringae pv. syringae induces systemic resistance to Pyricularia oryzae in rice. Physiol Mol Plant Pathol 39: 451-461

    Google Scholar 

  • Sticher L, Mauch-Mani B and Métraux J-P (1997) Systemic acquired resistance. Annu Rev Phytopathol 35: 235-270

    Google Scholar 

  • Uknes S, Mauch-Mani B, Moyer M, Potter S, Williams S, Dincher S, Chandler D, Slusarenko A, Ward E and Ryals J (1992) Acquired resistance in Arabidopsis. Plant Cell 4: 645-656

    Google Scholar 

  • Ward E, Uknes SJ, Williams SC, Dincher SS, Wiederhold DL, Alexander DC, AhI-Goy P, Métraux J-P and Ryals J (1991) Coordinate gene activity in response to agents that induce systemic acquired resistance. Plant Cell 3: 1085-1094

    Google Scholar 

  • Wäspi U, Misteli B, Hasslacher M, Jandrositz A, Kohlwein SD, Schwab H and Dudler R (1998) The defence-related rice gene Pir7b encodes an α/β hydrolase fold protein exhibiting esterase activity towards naphthol AS-esters. Eur J Biochem 254: 32-37

    Google Scholar 

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Author notes

  1. Ulrich Schaffrath

    Present address: Institute of Biology III, RWTH Aachen, D-52056, Aachen, Germany

Authors and Affiliations

  1. Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008, Zürich, Switzerland (Phone

    Patrick Schweizer, Edith Schlagenhauf, Ulrich Schaffrath & Robert Dudler

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  1. Patrick Schweizer
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  2. Edith Schlagenhauf
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  3. Ulrich Schaffrath
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  4. Robert Dudler
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Schweizer, P., Schlagenhauf, E., Schaffrath, U. et al. Different Patterns of Host Genes are Induced in Rice by Pseudomonas syringae, a Biological Inducer of Resistance, and the Chemical Inducer Benzothiadiazole (BTH). European Journal of Plant Pathology 105, 659–665 (1999). https://doi.org/10.1023/A:1008791223608

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