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Location of Prunus Necrotic Ringspot Ilarvirus Within Pollen Grains of Infected Nectarine Trees: Evidence from RT-PCR, Dot-blot and in situ Hybridisation

European Journal of Plant Pathology volume 105, pages 623–627 (1999)Cite this article

Abstract

Prunus necrotic ringspot Ilarvirus (PNRSV) is a pollen and seed-borne ilarvirus affecting most Prunus spp. The location of the virus in infected nectarine pollen grains was investigated by molecular hybridisation, RT-PCR and in situ hybridisation. The first two approaches revealed an internal location of the virus. In situ hybridisation demonstrated the virus in the bicellular pollen grain, where it was present in the cytoplasm of the vegetative cell but not in the generative cell. This result seems to indicate that the sperm cells, formed by the mitosis of the generative cell, are not involved in virus transmission to seed. Other possible mechanisms are discussed.

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Authors and Affiliations

  1. Departamento de Mejora y Patología Vegetal, CEBAS (CSIC), Apartado de Correos 4195, 30080, Murcia, Spain

    Federico Aparicio, Maria A. Sánchez-Pina, Jesus A. Sánchez-Navarro & Vicente Pallás

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  1. Federico Aparicio
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  2. Maria A. Sánchez-Pina
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  3. Jesus A. Sánchez-Navarro
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  4. Vicente Pallás
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Correspondence to Vicente Pallás.

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Aparicio, F., Sánchez-Pina, M.A., Sánchez-Navarro, J.A. et al. Location of Prunus Necrotic Ringspot Ilarvirus Within Pollen Grains of Infected Nectarine Trees: Evidence from RT-PCR, Dot-blot and in situ Hybridisation. European Journal of Plant Pathology 105, 623–627 (1999). https://doi.org/10.1023/A:1008783705183

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  • DOI: https://doi.org/10.1023/A:1008783705183

  • anthers
  • ilarvirus
  • PNRSV
  • pollen transmission