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European Journal of Plant Pathology

, Volume 105, Issue 6, pp 617–621 | Cite as

Potato Virus Y from Petunia can cause Symptoms of Potato Tuber Necrotic Ringspot Disease (PTNRD)

  • Neil BoonhamEmail author
  • Matthew Hims
  • Ian Barker
  • Nicola Spence
Article

Abstract

A potyvirus known to be an important agent involved in causing a disease of trailing petunias, was identified as being a member of the necrotic strain of potato virus Y (PVY) using a number of monoclonal antibodies. The sequence of the coat protein gene for the PVY isolate was determined and when compared with sequences for other PVY strains it was shown to cluster closely with isolates of PVYNTN and to have a recombination point present within the coat protein common with other isolates of PVYNTN. When inoculated onto potato tuber necrotic ringspot disease (PTNRD) susceptible potato cultivars the petunia isolate was found to be capable of causing necrotic tuber symptoms, consistent with those caused by other isolates of PVYNTN. Due to the number of similarities it is thought the petunia isolate belongs to the PVYNTN group of isolates. Out of 24 species of bedding and pot plant crops tested, 19 were shown by mechanical inoculation to be susceptible to PVY, highlighting not only a clear risk to a number of commercially important plant species from PVYNTN infected trailing petunias, but also other susceptible crops grown in these areas.

PVY PVYNTN potyvirus PTNRD 

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References

  1. Beczner L, Horvath H, Romhanyi I and Forster H (1984) Studies on the aetiology of tuber necrotic ringspot disease in potato. Potato Research 27: 339-352Google Scholar
  2. Bellardi MG, Rubiesautonell C and Vicchi V (1996) Virus infection of sufinia in Italy. Acta Hort 432: 306-310Google Scholar
  3. Blanco-Urgoiti B, Sanchez F, DeSanRoman C, Dopiazo J and Ponz F (1998) Potato virus Y group C isolates are homogeneous pathotype but two different genetic strains. Journal of General Virology 79: 2037-2042Google Scholar
  4. Boonham N and Barker I (1998) Strain specific recombinant antibodies to potato virus Y potyvirus. Journal of Virological Methods 74: 193-199Google Scholar
  5. Bravo-Almonacid F and Mentaberry AN (1989) Nucleotide cDNA sequence coding for the PVYO coat protein. Nucleic Acids Research 17: 4401Google Scholar
  6. Campos G (1995) CSL issue early Surfina alert. Grower 13: 4Google Scholar
  7. Chachulska AM, Chrzanowska M, Robaglia C and Zagorski W (1997) Tobacco veinal necrosis determinants are unlikely to be located within the 5′ and 3′ terminal sequences of the potato virus Y genome. Archives of Virology 142: 765-779Google Scholar
  8. Clark MF and Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34: 475-483Google Scholar
  9. Cockerham G (1970) Genetical studies on resistance to potato viruses X and Y. Heredity, 25: 309-348Google Scholar
  10. De Bokx JA and Huttinga H (1981) Potato virus Y. CMI/AAB Descriptions of plant viruses, 242Google Scholar
  11. Dhar AK and Singh RP (1997) Molecular characterization of coat protein genes of serologically distinct strains of potato virus Y necrotic strain (PVYN). Can J Microbiol 43: 677-683Google Scholar
  12. Edwardson and Christie (1991) The potyvirus group Vol 1–4, Florida Ag. Sta. Monograph 16Google Scholar
  13. Felsenstein J (1989) PHYLIP-phylogeny inference package (version 3.2). Cladistics 5: 164-166Google Scholar
  14. Hataya T, Inoue AK, Ohshima K and Shikata E (1994) Characterization and strain identification of a potato virus Y isolate non-reactive with monoclonal antibodies specific to the ordinary and necrotic strains. Intervirology 37: 12-19Google Scholar
  15. Hay JM, Fellowes AP and Timmerman GM (1989) Nucleotide sequence of the coat protein gene of a necrotic strain of potato virus Y from New Zealand. Archives of Virology 107: 111-122Google Scholar
  16. Hull R (1984) Rapid diagnosis of plant virus infections by spot hybridisation. Trends in Biotechnology 2: 2Google Scholar
  17. Le Romancer M and Nedellec M (1997) Effect of plant genotype, virus isolate and temperature on the expression of the potato tuber necrotic ring disease (PTNRD). Plant Pathology 46: 104-111Google Scholar
  18. Lesemann DE (1996) Viruses recently detected in vegetatively propagated petunia. Acta Hort 432: 88-94Google Scholar
  19. Revers F, Legall O, Candresse, T, Le Romancer M and Dunez J (1996) Frequent occurrence of recombinant potyvirus isolates. Journal of General Virology 77: 1953-1965Google Scholar
  20. Sharp C (1994) Potato virus YN (PVYN) in petunia Surfinia. Plant Disease Notice No 79. Ministry of Agriculture, Fisheries and Food, UKGoogle Scholar
  21. Shukla DD, Ward CW and Brunt AA (1994) The Potyviridae. Wallingford: CAB InternationalGoogle Scholar
  22. Sigvald R (1984) The relative efficiency of some aphid species as vectors of potato virus Y. Potato Research 27: 285-290Google Scholar
  23. Spence N, Barker I, O'Neill T and Wright D (1996) Viruses in trailing petunias. HDC Fact Sheet 04/96Google Scholar
  24. Thole V, Dalmay T, Burgyan J and Balazs E (1993) Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA. Gene 123: 149-156Google Scholar
  25. Vance VB, Moore D, Turpen TH, Bracker A and Hollowell VC (1992) The complete nucleotide sequence of pepper mottle virus genomic RNA: Comparison of the encoded polyprotein with those of other sequenced potyviruses. Virology 191: 19-30Google Scholar
  26. VanDenHeuvel JFJM, Vandervlught RAA, Verbeek M, Dehaan PT and Huttinga H (1994) Characteristics of a resistance breaking isolate of potato virus Y causing potato tuber necrotic ringspot disease. European Journal of Plant Pathology 100: 347-356Google Scholar
  27. Weidemann HL and Maiss E (1996) Detection of the potato tuber necrotic ringspot strain of potato virus Y (PVYNTN) by reverse transcription and immunocapture polymerase chain reaction. Journal of Plant Diseases and Protection 103: 337-345Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Neil Boonham
    • 1
    Email author
  • Matthew Hims
    • 1
  • Ian Barker
    • 1
  • Nicola Spence
    • 2
  1. 1.Central Science Laboratory, Sand HuttonYorkENGLAND; (E-mail
  2. 2.Horticulture Research International, WellesbourneWarwickEngland

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