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A Laboratory Assay for Phytophthora infestans Resistance in Various Solanum Species Reflects the Field Situation

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Abstract

Physiological and molecular research on resistance responses of Solanum tuberosum cultivars and partially resistant Solanum species to Phytophthora infestans requires a reliable resistance test that can be used in the laboratory. Laboratory tests performed on detached leaves and intact plants were compared with field tests for similarity of late blight reactions. Detached leaves from field-grown plants were as resistant as detached leaves from climate chamber-grown plants when challenged with P. infestans. However, detached leaves incubated in covered trays at high relative humidity were more susceptible than detached leaves kept in open trays or leaves on intact plants. The incubation conditions of detached leaves in covered trays rather than detachment itself appeared to affect the resistance expression. Detached leaves of some wild Solanum genotypes became partially infected, whereas intact plants were completely resistant when inoculated. Inoculation of leaves on intact plants, however, resulted in lower infection efficiencies. These limitations should be taken into account when choosing the appropriate inoculation method for specific purposes. For resistance screening, laboratory tests proved to be a good alternative for field tests. The ranking of resistance levels for twenty plant genotypes was similar under laboratory and field conditions.

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References

  • Beckman KB, Harrison JG and Ingram DS (1994) Optimization of a polyclonal enzyme-linked immunosorbent assay (ELISA) of fungal biomass for use in studies of plant defence responses. Physiological and Molecular Plant Pathology 44: 19-32

    Google Scholar 

  • Birhman RK and Singh BP (1995) Path-coefficient analyses and genetic parameters of the components of field resistance of potatoes to late blight. Annals of Applied Biology 127: 353-362

    Google Scholar 

  • Caten CE and Jinks JL (1968) Spontaneous variability of single isolates of Phytophthora infestans I. Cultural variation. Canadian Journal of Botany 46: 329-347

    Google Scholar 

  • Colon LT and Budding DJ (1988) Resistance to late blight (Phytophthora infestans) in ten wild Solanum species. Euphytica Supplement: 77-86

  • Colon LT, Budding DJ and Hoogendoorn J (1995a) Breeding for foliar resistance to Phytophthora infestans in potato: the influence of test conditions on the results of screening for field resistance. Dowley L, Bannon E, Cooke L, Keane T and O'Sullivan E. Phytophthora infestans 150: 282-288. Boole Press Ltd, Dublin

    Google Scholar 

  • Colon LT, Budding DJ, Keizer LCP and Pieters MJJ (1995b) Components of resistance to late blight (Phytophthora infestans) in eight South American Solanum species. European Journal of Plant Pathology 101: 441-456

    Google Scholar 

  • Colon LT, Turkensteen LJ, Prummel W, Budding DJ and Hoogendoorn J (1995c) Durable resistance to late blight (Phytophthora infestans) in old potato cultivars. European Journal of Plant Pathology 101: 387-397

    Google Scholar 

  • Dorrance AE and Inglis DA (1997) Assessment of greenhouse and laboratory screening methods for evaluating potato foliage for resistance to late blight. Plant Disease 81: 1206-1213

    Google Scholar 

  • Eijlander R and Stiekema WJ (1994) Biological containment of potato (Solanum tuberosum): Outcrossing to the related wild species black nightshade (Solanum nigrum) and bittersweet (Solanum dulcamara). Sexual Plant Reproduction 7: 29-40

    Google Scholar 

  • Fry WE (1978) Quantification of general resistance of potato cultivars and fungicide effects for integrated control of potato late blight. Phytopathology 68: 1650-1655

    Google Scholar 

  • Genstat 5 Committee (1987) Genstat 5 Reference Manual. Clarendon Press, Oxford

    Google Scholar 

  • Harrison J, Barker H, Lowe R and Rees E (1990) Estimation of amounts of Phytophthora infestans mycelium in leaf tissue by enzyme-linked immunoabsorbent assay. Plant Pathology 39: 274-277

    Google Scholar 

  • Hermsen JGT and Ramanna MS (1973) Double-bridge hybrids of Solanum bulbocastanum and cultivars of Solanum tuberosum. Euphytica 22: 457-466

    Google Scholar 

  • Hodgson W (1961) Laboratory testing of the potato for partial resistance to Phytophthora infestans. American Potato Journal 38: 261-264

    Google Scholar 

  • Hodgson W (1962) Studies on the nature of partial resistance in the potato to Phytophthora infestans. American Potato Journal 39: 8-13

    Google Scholar 

  • Kamoun S, van West P and Govers F (1998) Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing beta-glucoronidase. European Journal of Plant Pathology 104: 521-525

    Google Scholar 

  • Knutson K (1962) Studies on the nature of field resistance of the potato to late blight. American Potato Journal 39: 152-162

    Google Scholar 

  • Lapwood D (1961) Laboratory assessments of the susceptibility of potato haulm to blight (Phytophthora infestans). European Potato Journal 4: 117-127

    Google Scholar 

  • Malcolmson J (1969) Factors involved in resistance to blight (Phytophthora infestans (Mont.) de Bary) in potatoes and assessment of resistance using detached leaves. Annals of Applied Biology 64: 461-468

    Google Scholar 

  • Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497

    Google Scholar 

  • Shaner G and Finney RE (1977) The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology 67: 1051-1056

    Google Scholar 

  • Singh BP and Birhman RK (1994) Laboratory estimation of field resistance of potato to late blight. Journal of Phytopathology 140: 71-76

    Google Scholar 

  • Stewart HE, Flavelle PH, McCalmont DC and Wastie RL (1983) Correlation between glasshouse and field tests for resistance to foliage blight caused by Phytophthora infestans. Potato Research 26: 41-48

    Google Scholar 

  • Umaerus V (1969) Studies on field resistance to Phytophthora infestans. 5. Mechanisms of resistance and applications to potato breeding. Zeitschrift für Pflanzenzüchtung 63: 1-23

    Google Scholar 

  • Umaerus V and Lihnell D (1976) A laboratory method for measuring the degree of attack by Phytophthora infestans. Potato Research 19: 91-107

    Google Scholar 

Download references

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Vleeshouwers, V.G., van Dooijeweert, W., Paul Keizer, L. et al. A Laboratory Assay for Phytophthora infestans Resistance in Various Solanum Species Reflects the Field Situation. European Journal of Plant Pathology 105, 241–250 (1999). https://doi.org/10.1023/A:1008710700363

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