Abstract
RFLPs of chloroplast (cp) and mitochondrial (mt) DNA have been used to characterize the cytoplasmic genome of wild soybean, Glycine soja, growing in China. We have found from the RFLPs of that the cytoplasmic genomes of most Chinese wild soybeans have a combination of cpIII with mt-a or a combination of cpII with mt-b accompanied by mtIV or mtV according to our classification. CpII was not observed in combination with mt-a and cpIII was not observed in combination with mt-b. The regional distribution of these two types showed clines with opposite directions. The Yangtze River Valley had the greatest diversity in each of cp and two mt profiles detected in this study and the cytoplasmic genome combining these three profiles showed the highest degree of polymorphism in this region. The Yangtze River Valley may be a center of cytoplasmic diversity of wild soybean and may contain various genetic resources of soybean.
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Close, P.S., R.C. Shoemaker & P. Keim, 1989. Distribution of restriction site polymorphism within the chloroplast genome of the genus Glycine, subgenus Soja. Theor. Appl. Genet. 77: 768–776.
Corriveau, J.L. & A.W. Coleman, 1988. Cytological evidence for maternal inheritance of plastid DNA in the genus Glycine subgenera Glycine and Soja.Soybean Genet. Newsl. 15: 89–92.
Doyle, J.J. & J.L. Doyle, 1990. Isolation of plant DNA from fresh tissue. FOCUS 12: 13-15.
Grabau, E.A., W.H. Davis, N.D. Phelps & B.G. Gengenbach, 1989. Restriction fragment length polymorphism in subclass of the 'Mandarin' soybean cytoplasm. Crop Sci. 29: 1554–1559.
Grabau, E.A., W.H. Davis, N.D. Phelps & B.G. Gengenbach, 1992. Classification of soybean cultivars based on mitochondrial DNA restriction fragment polymorphisms. Crop Sci. 32: 271–274.
Grabau, E.A. & W.H. Davis, 1992. Cytoplasmic diversity in Glycine soja. Soybean Genet. Newsl. 19: 140–144.
Hanlon, R. & E.A. Grabau, 1995. Cytoplasmic diversity in old domestic varieties of soybean using two mitochondrial markers. Crop Sci. 35: 1148–1151.
Hatfield, P.M., R.C. Shoemaker & R.G. Palmer, 1985. Maternal inheritance of chloroplast DNA within the genus Glycine subgenus Soja. J. Hered. 76: 373–374.
Hirata, T., J. Abe & Y. Shimamoto, 1996. RFLPs of chloroplast and mitochondrial genomes in summer and autumn maturing cultivar groups of soybean in Kyushu district of Japan. Soybean Genet. Newsl. 23: 107–111.
Hymowitz, T., 1970. On the domestication of the soybean. Econ. Bot. 23: 408–421.
Hymowitz, T. & C.A. Newell, 1981. Taxonomy of the genus Glycine, domestication and uses of soybeans. Econ. Bot. 35: 272–288.
Kishima, Y., 1988. Molecular and phylogenic studies on the organelle genome variations among sugar beet (Beta vulgaris L.) and its related species. PhD. thesis, Hokkaido Univ., pp. 22–37 (in Japanese).
Shimamoto, Y., A. Hasegawa, J. Abe, M. Ohara & T. Mikami, 1992. Glycine soja germplasm in Japan: Isozyme and chloroplast DNA variation. Soybean Genet. Newsl. 19: 73–77.
Shoemaker, R.C., P.M. Hatfield, R.G. Palmer & A.G. Atherly, 1986. Chloroplast DNA variation in the genus Glycine subgenus Soja. J. Hered. 77: 26–30.
Sisson, V.A., C.A. Brim & C.S. Levings, III, 1978. Characterization of cytoplasmic diversity in soybean by restriction endonuclease analysis. Crop Sci. 18: 991–996.
Tozuka, A., H. Fukushi, T. Hirata, M. Ohara, A. Kanazawa, T. Mikami, J. Abe & Y. Shimamoto, 1998. Composite and clinal distribution of Glycine soja in Japan revealed by RFLP analysis of mitochondrial DNA. Theor. Appl. Genet. 96: 170–176.
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Shimamoto, Y., Fukushi, H., Abe, J. et al. RFLPs of chloroplast and mitochondrial DNA in wild soybean, Glycine soja, growing in China. Genetic Resources and Crop Evolution 45, 433–439 (1998). https://doi.org/10.1023/A:1008693603526
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DOI: https://doi.org/10.1023/A:1008693603526